| BOUCHAL, Pavel, Zbyněk ZDRÁHAL, Šárka HELÁNOVÁ, Oldřich JANICZEK, Kevin B. HALLBERG and Martin MANDL. Proteomic and bioinformatic analysis of iron- and sulfur-oxidizing Acidithiobacillus ferrooxidans using immobilized pH gradients and mass spectrometry. Proteomics. Weinheim: Wiley-VCH, 2006, vol. 6, No 15, p. 4278-4285. ISSN 1615-9853. |
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@article{637604,
author = {Bouchal, Pavel and Zdráhal, Zbyněk and Helánová, Šárka and Janiczek, Oldřich and Hallberg, Kevin B. and Mandl, Martin},
article_location = {Weinheim},
article_number = {15},
keywords = {Acidithiobacilus ferrooxidans; Iron oxidation; Protein reduction; Proteome; Sulfur oxidation},
language = {eng},
issn = {1615-9853},
journal = {Proteomics},
title = {Proteomic and bioinformatic analysis of iron- and sulfur-oxidizing Acidithiobacillus ferrooxidans using immobilized pH gradients and mass spectrometry},
url = {http://www3.interscience.wiley.com/cgi-bin/fulltext/112679635/PDFSTART},
volume = {6},
year = {2006}
}
TY - JOUR
ID - 637604
AU - Bouchal, Pavel - Zdráhal, Zbyněk - Helánová, Šárka - Janiczek, Oldřich - Hallberg, Kevin B. - Mandl, Martin
PY - 2006
TI - Proteomic and bioinformatic analysis of iron- and sulfur-oxidizing Acidithiobacillus ferrooxidans using immobilized pH gradients and mass spectrometry
JF - Proteomics
VL - 6
IS - 15
SP - 4278-4285
EP - 4278-4285
PB - Wiley-VCH
SN - 16159853
KW - Acidithiobacilus ferrooxidans
KW - Iron oxidation
KW - Protein reduction
KW - Proteome
KW - Sulfur oxidation
UR - http://www3.interscience.wiley.com/cgi-bin/fulltext/112679635/PDFSTART
N2 - A comparative analysis of the protein composition of Acidithiobacillus ferrooxidans cells grown on elemental sulfur and ferrous iron was performed. A newly developed protocol involving immobilized pH gradients, improved protein reduction, mass spectrometry protein identification and full genome sequence information was applied. This approach resulted in more than 1300 protein spots displayed in broad and basic pH ranges, the best At. ferrooxidans proteome resolution to date. A comparative image analysis revealed that the proteome was significantly influenced by the growth type, and allowed for the detection of many physiologically important proteins. Among them were sulfate adenylyltransferase and sulfide dehydrogenase, which are involved in sulfate assimilation and sulfide metabolism, respectively. Many other proteins were related to important processes like cell attachment and electron transport. Co-migration of phosphate and sulfate transport proteins was also observed.
ER -
BOUCHAL, Pavel, Zbyněk ZDRÁHAL, Šárka HELÁNOVÁ, Oldřich JANICZEK, Kevin B. HALLBERG and Martin MANDL. Proteomic and bioinformatic analysis of iron- and sulfur-oxidizing Acidithiobacillus ferrooxidans using immobilized pH gradients and mass spectrometry. \textit{Proteomics}. Weinheim: Wiley-VCH, 2006, vol.~6, No~15, p.~4278-4285. ISSN~1615-9853.
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