BOUCHAL, Pavel, Zbyněk ZDRÁHAL, Šárka HELÁNOVÁ, Oldřich JANICZEK, Kevin B. HALLBERG and Martin MANDL. Proteomic and bioinformatic analysis of iron- and sulfur-oxidizing Acidithiobacillus ferrooxidans using immobilized pH gradients and mass spectrometry. Proteomics. Weinheim: Wiley-VCH, 2006, vol. 6, No 15, p. 4278-4285. ISSN 1615-9853. |
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@article{637604, author = {Bouchal, Pavel and Zdráhal, Zbyněk and Helánová, Šárka and Janiczek, Oldřich and Hallberg, Kevin B. and Mandl, Martin}, article_location = {Weinheim}, article_number = {15}, keywords = {Acidithiobacilus ferrooxidans; Iron oxidation; Protein reduction; Proteome; Sulfur oxidation}, language = {eng}, issn = {1615-9853}, journal = {Proteomics}, title = {Proteomic and bioinformatic analysis of iron- and sulfur-oxidizing Acidithiobacillus ferrooxidans using immobilized pH gradients and mass spectrometry}, url = {http://www3.interscience.wiley.com/cgi-bin/fulltext/112679635/PDFSTART}, volume = {6}, year = {2006} }
TY - JOUR ID - 637604 AU - Bouchal, Pavel - Zdráhal, Zbyněk - Helánová, Šárka - Janiczek, Oldřich - Hallberg, Kevin B. - Mandl, Martin PY - 2006 TI - Proteomic and bioinformatic analysis of iron- and sulfur-oxidizing Acidithiobacillus ferrooxidans using immobilized pH gradients and mass spectrometry JF - Proteomics VL - 6 IS - 15 SP - 4278-4285 EP - 4278-4285 PB - Wiley-VCH SN - 16159853 KW - Acidithiobacilus ferrooxidans KW - Iron oxidation KW - Protein reduction KW - Proteome KW - Sulfur oxidation UR - http://www3.interscience.wiley.com/cgi-bin/fulltext/112679635/PDFSTART N2 - A comparative analysis of the protein composition of Acidithiobacillus ferrooxidans cells grown on elemental sulfur and ferrous iron was performed. A newly developed protocol involving immobilized pH gradients, improved protein reduction, mass spectrometry protein identification and full genome sequence information was applied. This approach resulted in more than 1300 protein spots displayed in broad and basic pH ranges, the best At. ferrooxidans proteome resolution to date. A comparative image analysis revealed that the proteome was significantly influenced by the growth type, and allowed for the detection of many physiologically important proteins. Among them were sulfate adenylyltransferase and sulfide dehydrogenase, which are involved in sulfate assimilation and sulfide metabolism, respectively. Many other proteins were related to important processes like cell attachment and electron transport. Co-migration of phosphate and sulfate transport proteins was also observed. ER -
BOUCHAL, Pavel, Zbyněk ZDRÁHAL, Šárka HELÁNOVÁ, Oldřich JANICZEK, Kevin B. HALLBERG and Martin MANDL. Proteomic and bioinformatic analysis of iron- and sulfur-oxidizing Acidithiobacillus ferrooxidans using immobilized pH gradients and mass spectrometry. \textit{Proteomics}. Weinheim: Wiley-VCH, 2006, vol.~6, No~15, p.~4278-4285. ISSN~1615-9853.
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