A suggestion of enzymatic biosensor for determination of
chlorinated pesticides

D 2006

A suggestion of enzymatic biosensor for determination of chlorinated pesticides

MIKELOVÁ, Radka, Zbyněk PROKOP, Jan HRADECKÝ, Libuše TRNKOVÁ, Vojtěch ADAM et. al.

Basic information

Original name

A suggestion of enzymatic biosensor for determination of chlorinated pesticides

Name in Czech

Návrh enzymatického biosenzoru pro detekci chlorovaných pesticidů

Authors

MIKELOVÁ, Radka (203 Czech Republic), Zbyněk PROKOP (203 Czech Republic), Jan HRADECKÝ (203 Czech Republic), Libuše TRNKOVÁ (203 Czech Republic, guarantor), Vojtěch ADAM (203 Czech Republic), Jiří DAMBORSKÝ (203 Czech Republic) and René KIZEK (203 Czech Republic)

Edition

první. Bordeaux, 11th International conference on electroanalysis, p. 1-45, 300 pp. 2006

Publisher

European Society for ElectroAnalytical Chemistry

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10405 Electrochemistry

Country of publisher

France

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/06:00020011

Organization unit

Faculty of Science

Keywords in English

silver electrode; carbon past electrode; modification; chloride; chlorinated hydrocarbon; electrochemistry

Abstract

ORIG CZ

V originále

Introduction. Chlorinated pesticides such as derivates of DTT, hexachlorobenzene and many others have been used to protect of plants. It is a common knowledge that residues of these pesticides deposit in adipose tissues at animals and could be transferred from here to eggs or to breast milk of mammals. A higher concentration of these compounds could be responsible for infertility and for tumour disease. Therefore a development of simple and accurate tool for detection of them is need. Objective. The aim of this work was to suggest a biosensor used for determination of chlorinated pesticides. Materials and methods. Stationary electrochemical measurements were performed with AUTOLAB Analyser connected to VA-Stand 663, using a standard cell with three electrodes. The working electrode was carbon paste electrode. A flow injection analysis coupled with electrochemical detector consisted of solvent delivery pump, a guard cell, a reaction coil (1 m) and an electrochemical detector. The electrochemical detector includes one low volume flow-through analytical cells, which is consisted of glassy carbon working electrode, palladium electrode as reference electrode and auxiliary carbon electrode, and Coulochem III as a control module. Results. Our suggested biosensor is based on a determination of chlorides on carbon paste and/or glassy carbon electrode both in stationary and flow system. We improved sensitivity and selectivity of the analysis by stationary electrochemical system, particularly by carbon past electrode, through modification of the electrode by silver nitrate. We were able to determine chlorides in units of 10-8 M due to this modification. After that we evaluated that we were able to determine chloride, we suggested our biosensor, which were consisted from the electrode and a dehalogenases. The enzymes were capable of dechlorinating chlorinated hydrocarbons. We applied the biosensor to detect a 1-chlorohexane and were successful. Moreover, we attempted to determine chlorinated hydrocarbons by flow technique with electrochemical detection. Conclusions. Commonly used methods for determination of chlorinated pesticides are time consuming and highly cost. Here, we suggested a technique for simple and low cost determination of these compounds.

In Czech

Návrh enzymatického biosenzoru pro detekci chlorovaných pesticidů

Links

GP525/04/P132, research and development project

Name: Studium obranných mechanismů rostlin při stresu způsobeném těžkými kovy

MSM0021622412, plan (intention)

Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)

Citovat

MIKELOVÁ, Radka, Zbyněk PROKOP, Jan HRADECKÝ, Libuše TRNKOVÁ, Vojtěch ADAM, Jiří DAMBORSKÝ and René KIZEK. A suggestion of enzymatic biosensor for determination of chlorinated pesticides. In 11th International conference on electroanalysis. první. Bordeaux: European Society for ElectroAnalytical Chemistry, 2006, p. 1-45, 300 pp.

@inproceedings{637914,
   author = {Mikelová, Radka and Prokop, Zbyněk and Hradecký, Jan and Trnková, Libuše and Adam, Vojtěch and Damborský, Jiří and Kizek, René},
   address = {Bordeaux},
   booktitle = {11th International conference on electroanalysis},
   edition = {první},
   keywords = {silver electrode; carbon past electrode; modification; chloride; chlorinated hydrocarbon; electrochemistry},
   language = {eng},
   location = {Bordeaux},
   pages = {1-45},
   publisher = {European Society for ElectroAnalytical Chemistry},
   title = {A suggestion of enzymatic biosensor for determination of chlorinated pesticides},
   year = {2006}
}

TY  - JOUR
ID  - 637914
AU  - Mikelová, Radka - Prokop, Zbyněk - Hradecký, Jan - Trnková, Libuše - Adam, Vojtěch - Damborský, Jiří - Kizek, René
PY  - 2006
TI  - A suggestion of enzymatic biosensor for determination of chlorinated pesticides
PB  - European Society for ElectroAnalytical Chemistry
CY  - Bordeaux
KW  - silver electrode
KW  - carbon past electrode
KW  - modification
KW  - chloride
KW  - chlorinated hydrocarbon
KW  - electrochemistry
N2  - Introduction. Chlorinated pesticides such as derivates of DTT, hexachlorobenzene and many others have been used to protect of plants. It is a common knowledge that residues of these pesticides deposit in adipose tissues at animals and could be transferred from here to eggs or to breast milk of mammals. A higher concentration of these compounds could be responsible for infertility and for tumour disease. Therefore a development of simple and accurate tool for detection of them is need. Objective. The aim of this work was to suggest a biosensor used for determination of chlorinated pesticides. Materials and methods. Stationary electrochemical measurements were performed with AUTOLAB Analyser connected to VA-Stand 663, using a standard cell with three electrodes. The working electrode was carbon paste electrode. A flow injection analysis coupled with electrochemical detector consisted of solvent delivery pump, a guard cell, a reaction coil (1 m) and an electrochemical detector. The electrochemical detector includes one low volume flow-through analytical cells, which is consisted of glassy carbon working electrode, palladium electrode as reference electrode and auxiliary carbon electrode, and Coulochem III as a control module. Results. Our suggested biosensor is based on a determination of chlorides on carbon paste and/or glassy carbon electrode both in stationary and flow system. We improved sensitivity and selectivity of the analysis by stationary electrochemical system, particularly by carbon past electrode, through modification of the electrode by silver nitrate. We were able to determine chlorides in units of 10-8 M due to this modification. After that we evaluated that we were able to determine chloride, we suggested our biosensor, which were consisted from the electrode and a dehalogenases. The enzymes were capable of dechlorinating chlorinated hydrocarbons. We applied the biosensor to detect a 1-chlorohexane and were successful. Moreover, we attempted to determine chlorinated hydrocarbons by flow technique with electrochemical detection. Conclusions. Commonly used methods for determination of chlorinated pesticides are time consuming and highly cost. Here, we suggested a technique for simple and low cost determination of these compounds.
ER  -

MIKELOVÁ, Radka, Zbyněk PROKOP, Jan HRADECKÝ, Libuše TRNKOVÁ, Vojtěch ADAM, Jiří DAMBORSKÝ and René KIZEK. A suggestion of enzymatic biosensor for determination of chlorinated pesticides. In \textit{11th International conference on electroanalysis}. první. Bordeaux: European Society for ElectroAnalytical Chemistry, 2006, p.~1-45, 300 pp.

Detailed Information on Publication Record