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@inproceedings{637917,
author = {Petrlová, Jitka and Trnková, Libuše and Adam, Vojtěch and Zehnálek, Josef and Zeman, Ladislav and Kizek, René},
address = {Bordeaux},
booktitle = {11th International conference on electroanalysis},
edition = {první},
keywords = {Metallothionein; apo-metallothionein; biosensor; electrochemistry; carcinogenesis; spontaneous mutagenesis; tumour;},
language = {eng},
location = {Bordeaux},
pages = {8-8},
publisher = {European Society for ElectroAnalytical Chemistry},
title = {Electrochemical investigation of metallothionein and apo-metallothionein},
year = {2006}
}
TY - JOUR
ID - 637917
AU - Petrlová, Jitka - Trnková, Libuše - Adam, Vojtěch - Zehnálek, Josef - Zeman, Ladislav - Kizek, René
PY - 2006
TI - Electrochemical investigation of metallothionein and apo-metallothionein
PB - European Society for ElectroAnalytical Chemistry
CY - Bordeaux
KW - Metallothionein
KW - apo-metallothionein
KW - biosensor
KW - electrochemistry
KW - carcinogenesis
KW - spontaneous mutagenesis
KW - tumour;
N2 - Introduction. Metallothionein (MT) belongs to group of intracellular, cysteine-rich proteins with molecular weight from 6 to 10 kDa, which are usually abundant at low content at human organisms. Their marked expression could be caused by many exogenous and endogenous factors. During last 15 years number of authors are has been concerning with study of MT involvement in carcinogenesis and spontaneous mutagenesis, because an increasing amount of MT in tumour cells probably relates with cell proliferation. On the base of the abovementioned reasons, it is necessary to develop a method that could be used not only for determination of MT but also for study of MT structural changes during stress conditions. Objective. The aim of this work was to study the effect of ethylendiamine-N, N, N`, N`-tetraacetic acid (EDTA) on MT structure by means of electrochemical techniques. Materials and methods. Electrochemical measurements were performed with AUTOLAB Analyser connected to VA-Stand 663, using a standard cell with three electrodes. The working electrode was a hanging mercury drop electrode. The reference electrode was Ag/AgCl/3M KCl electrode and the auxiliary electrode was the graphite electrode. The samples of the MT were reduced before each measurement. Results. It is known that EDTA is able to bind heavy metals not only from surrounding medium but also from structures of biological compounds. Here we added the different EDTA concentrations (up to 500 nM) to 100 nM MTCd5Zn2. The sample was analysed by adsorptive transfer stripping differential pulse voltammetry. On the base of the obtained results it follows that the signals, which correspond to reduction of zinc and cadmium present in MT structure, disappeared with increasing EDTA concentration. In addition we obtained the apo-MT (MT without heavy metal) at the highest concentration of EDTA (500 nM). The comparison between MT and apo-MT signals have been done and discussed. Conclusions. The described technique offers a good tool not only for study of heavy metal amounts in MT structure but also for characterization of conformation changes in MT structure.
ER -
PETRLOVÁ, Jitka, Libuše TRNKOVÁ, Vojtěch ADAM, Josef ZEHNÁLEK, Ladislav ZEMAN and René KIZEK. Electrochemical investigation of metallothionein and apo-metallothionein. In \textit{11th International conference on electroanalysis}. první. Bordeaux: European Society for ElectroAnalytical Chemistry, 2006, p.~8-8.
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