Electrochemical investigation of metallothionein and
apo-metallothionein

D 2006

Electrochemical investigation of metallothionein and apo-metallothionein

PETRLOVÁ, Jitka, Libuše TRNKOVÁ, Vojtěch ADAM, Josef ZEHNÁLEK, Ladislav ZEMAN et. al.

Basic information

Original name

Electrochemical investigation of metallothionein and apo-metallothionein

Name in Czech

Elektrochemická studie metalothioneinu a apo- metalothioneinu

Authors

PETRLOVÁ, Jitka (203 Czech Republic), Libuše TRNKOVÁ (203 Czech Republic, guarantor), Vojtěch ADAM (203 Czech Republic), Josef ZEHNÁLEK (203 Czech Republic), Ladislav ZEMAN (203 Czech Republic) and René KIZEK (203 Czech Republic)

Edition

první. Bordeaux, 11th International conference on electroanalysis, p. 8-8, 1 pp. 2006

Publisher

European Society for ElectroAnalytical Chemistry

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10405 Electrochemistry

Country of publisher

France

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/06:00020013

Organization unit

Faculty of Science

Keywords in English

Metallothionein; apo-metallothionein; biosensor; electrochemistry; carcinogenesis; spontaneous mutagenesis; tumour;

Abstract

ORIG CZ

V originále

Introduction. Metallothionein (MT) belongs to group of intracellular, cysteine-rich proteins with molecular weight from 6 to 10 kDa, which are usually abundant at low content at human organisms. Their marked expression could be caused by many exogenous and endogenous factors. During last 15 years number of authors are has been concerning with study of MT involvement in carcinogenesis and spontaneous mutagenesis, because an increasing amount of MT in tumour cells probably relates with cell proliferation. On the base of the abovementioned reasons, it is necessary to develop a method that could be used not only for determination of MT but also for study of MT structural changes during stress conditions. Objective. The aim of this work was to study the effect of ethylendiamine-N, N, N`, N`-tetraacetic acid (EDTA) on MT structure by means of electrochemical techniques. Materials and methods. Electrochemical measurements were performed with AUTOLAB Analyser connected to VA-Stand 663, using a standard cell with three electrodes. The working electrode was a hanging mercury drop electrode. The reference electrode was Ag/AgCl/3M KCl electrode and the auxiliary electrode was the graphite electrode. The samples of the MT were reduced before each measurement. Results. It is known that EDTA is able to bind heavy metals not only from surrounding medium but also from structures of biological compounds. Here we added the different EDTA concentrations (up to 500 nM) to 100 nM MTCd5Zn2. The sample was analysed by adsorptive transfer stripping differential pulse voltammetry. On the base of the obtained results it follows that the signals, which correspond to reduction of zinc and cadmium present in MT structure, disappeared with increasing EDTA concentration. In addition we obtained the apo-MT (MT without heavy metal) at the highest concentration of EDTA (500 nM). The comparison between MT and apo-MT signals have been done and discussed. Conclusions. The described technique offers a good tool not only for study of heavy metal amounts in MT structure but also for characterization of conformation changes in MT structure.

In Czech

Elektrochemická studie metalothioneinu a apo- metalothioneinu

Links

GP525/04/P132, research and development project

Name: Studium obranných mechanismů rostlin při stresu způsobeném těžkými kovy

MSM0021622412, plan (intention)

Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)

Citovat

PETRLOVÁ, Jitka, Libuše TRNKOVÁ, Vojtěch ADAM, Josef ZEHNÁLEK, Ladislav ZEMAN and René KIZEK. Electrochemical investigation of metallothionein and apo-metallothionein. In 11th International conference on electroanalysis. první. Bordeaux: European Society for ElectroAnalytical Chemistry, 2006, p. 8-8.

@inproceedings{637917,
   author = {Petrlová, Jitka and Trnková, Libuše and Adam, Vojtěch and Zehnálek, Josef and Zeman, Ladislav and Kizek, René},
   address = {Bordeaux},
   booktitle = {11th International conference on electroanalysis},
   edition = {první},
   keywords = {Metallothionein; apo-metallothionein; biosensor; electrochemistry; carcinogenesis; spontaneous mutagenesis; tumour;},
   language = {eng},
   location = {Bordeaux},
   pages = {8-8},
   publisher = {European Society for ElectroAnalytical Chemistry},
   title = {Electrochemical investigation of metallothionein and apo-metallothionein},
   year = {2006}
}

TY  - JOUR
ID  - 637917
AU  - Petrlová, Jitka - Trnková, Libuše - Adam, Vojtěch - Zehnálek, Josef - Zeman, Ladislav - Kizek, René
PY  - 2006
TI  - Electrochemical investigation of metallothionein and apo-metallothionein
PB  - European Society for ElectroAnalytical Chemistry
CY  - Bordeaux
KW  - Metallothionein
KW  - apo-metallothionein
KW  - biosensor
KW  - electrochemistry
KW  - carcinogenesis
KW  - spontaneous mutagenesis
KW  - tumour;
N2  - Introduction. Metallothionein (MT) belongs to group of intracellular, cysteine-rich proteins with molecular weight from 6 to 10 kDa, which are usually abundant at low content at human organisms. Their marked expression could be caused by many exogenous and endogenous factors. During last 15 years number of authors are has been concerning with study of MT involvement in carcinogenesis and spontaneous mutagenesis, because an increasing amount of MT in tumour cells probably relates with cell proliferation. On the base of the abovementioned reasons, it is necessary to develop a method that could be used not only for determination of MT but also for study of MT structural changes during stress conditions. Objective. The aim of this work was to study the effect of ethylendiamine-N, N, N`, N`-tetraacetic acid (EDTA) on MT structure by means of electrochemical techniques. Materials and methods. Electrochemical measurements were performed with AUTOLAB Analyser connected to VA-Stand 663, using a standard cell with three electrodes. The working electrode was a hanging mercury drop electrode. The reference electrode was Ag/AgCl/3M KCl electrode and the auxiliary electrode was the graphite electrode. The samples of the MT were reduced before each measurement. Results. It is known that EDTA is able to bind heavy metals not only from surrounding medium but also from structures of biological compounds. Here we added the different EDTA concentrations (up to 500 nM) to 100 nM MTCd5Zn2. The sample was analysed by adsorptive transfer stripping differential pulse voltammetry. On the base of the obtained results it follows that the signals, which correspond to reduction of zinc and cadmium present in MT structure, disappeared with increasing EDTA concentration. In addition we obtained the apo-MT (MT without heavy metal) at the highest concentration of EDTA (500 nM). The comparison between MT and apo-MT signals have been done and discussed. Conclusions. The described technique offers a good tool not only for study of heavy metal amounts in MT structure but also for characterization of conformation changes in MT structure.
ER  -

PETRLOVÁ, Jitka, Libuše TRNKOVÁ, Vojtěch ADAM, Josef ZEHNÁLEK, Ladislav ZEMAN and René KIZEK. Electrochemical investigation of metallothionein and apo-metallothionein. In \textit{11th International conference on electroanalysis}. první. Bordeaux: European Society for ElectroAnalytical Chemistry, 2006, p.~8-8.

Detailed Information on Publication Record