BENEŠ, Petr, Vendula MACEČKOVÁ, Zbyněk ZDRÁHAL, Hana KONEČNÁ, Eva ZAHRADNÍČKOVÁ, Jan MUŽÍK and Jan ŠMARDA. Role of vimentin in regulation of monocyte/macrophage differentiation. Differentiation. Malden, USA: Blackwell Publishing, 2006, vol. 74, No 6, p. 265-276. ISSN 0301-4681. |
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@article{638386, author = {Beneš, Petr and Macečková, Vendula and Zdráhal, Zbyněk and Konečná, Hana and Zahradníčková, Eva and Mužík, Jan and Šmarda, Jan}, article_location = {Malden, USA}, article_number = {6}, keywords = {vimentin; differentiation; macrophage; phagocytosis; proliferation}, language = {eng}, issn = {0301-4681}, journal = {Differentiation}, title = {Role of vimentin in regulation of monocyte/macrophage differentiation}, volume = {74}, year = {2006} }
TY - JOUR ID - 638386 AU - Beneš, Petr - Macečková, Vendula - Zdráhal, Zbyněk - Konečná, Hana - Zahradníčková, Eva - Mužík, Jan - Šmarda, Jan PY - 2006 TI - Role of vimentin in regulation of monocyte/macrophage differentiation JF - Differentiation VL - 74 IS - 6 SP - 265-276 EP - 265-276 PB - Blackwell Publishing SN - 03014681 KW - vimentin KW - differentiation KW - macrophage KW - phagocytosis KW - proliferation N2 - We explored the identity and function of an unknown protein that was greatly overexpressed in v-myb-transformed BM2 monoblasts undergoing differentiation to macrophage-like cells. We identified this protein as vimentin, the intermediate filament protein. We show that an increased level of the vimentin protein results from activation of the vimentin gene promoter occurring in monoblastic cells induced to differentiate by multiple agents. Furthermore, our studies reveal that the vimentin gene promoter is stimulated by Myb and Jun proteins, the key transcriptional regulators of myeloid maturation. Silencing of vimentin gene expression using siRNA markedly suppressed the ability of BM2 cells to form macrophage polykaryons active in phagocytosis and producing reactive oxygen species. These findings document that up-regulation of vimentin gene expression is important for formation of fully active macrophage-like cells. ER -
BENEŠ, Petr, Vendula MACEČKOVÁ, Zbyněk ZDRÁHAL, Hana KONEČNÁ, Eva ZAHRADNÍČKOVÁ, Jan MUŽÍK and Jan ŠMARDA. Role of vimentin in regulation of monocyte/macrophage differentiation. \textit{Differentiation}. Malden, USA: Blackwell Publishing, 2006, vol.~74, No~6, p.~265-276. ISSN~0301-4681.
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