| BENEŠ, Petr, Vendula MACEČKOVÁ, Zbyněk ZDRÁHAL, Hana KONEČNÁ, Eva ZAHRADNÍČKOVÁ, Jan MUŽÍK and Jan ŠMARDA. Role of vimentin in regulation of monocyte/macrophage differentiation. Differentiation. Malden, USA: Blackwell Publishing, 2006, vol. 74, No 6, p. 265-276. ISSN 0301-4681. |
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@article{638386,
author = {Beneš, Petr and Macečková, Vendula and Zdráhal, Zbyněk and Konečná, Hana and Zahradníčková, Eva and Mužík, Jan and Šmarda, Jan},
article_location = {Malden, USA},
article_number = {6},
keywords = {vimentin; differentiation; macrophage; phagocytosis; proliferation},
language = {eng},
issn = {0301-4681},
journal = {Differentiation},
title = {Role of vimentin in regulation of monocyte/macrophage differentiation},
volume = {74},
year = {2006}
}
TY - JOUR
ID - 638386
AU - Beneš, Petr - Macečková, Vendula - Zdráhal, Zbyněk - Konečná, Hana - Zahradníčková, Eva - Mužík, Jan - Šmarda, Jan
PY - 2006
TI - Role of vimentin in regulation of monocyte/macrophage differentiation
JF - Differentiation
VL - 74
IS - 6
SP - 265-276
EP - 265-276
PB - Blackwell Publishing
SN - 03014681
KW - vimentin
KW - differentiation
KW - macrophage
KW - phagocytosis
KW - proliferation
N2 - We explored the identity and function of an unknown protein that was greatly overexpressed in v-myb-transformed BM2 monoblasts undergoing differentiation to macrophage-like cells. We identified this protein as vimentin, the intermediate filament protein. We show that an increased level of the vimentin protein results from activation of the vimentin gene promoter occurring in monoblastic cells induced to differentiate by multiple agents. Furthermore, our studies reveal that the vimentin gene promoter is stimulated by Myb and Jun proteins, the key transcriptional regulators of myeloid maturation. Silencing of vimentin gene expression using siRNA markedly suppressed the ability of BM2 cells to form macrophage polykaryons active in phagocytosis and producing reactive oxygen species. These findings document that up-regulation of vimentin gene expression is important for formation of fully active macrophage-like cells.
ER -
BENEŠ, Petr, Vendula MACEČKOVÁ, Zbyněk ZDRÁHAL, Hana KONEČNÁ, Eva ZAHRADNÍČKOVÁ, Jan MUŽÍK and Jan ŠMARDA. Role of vimentin in regulation of monocyte/macrophage differentiation. \textit{Differentiation}. Malden, USA: Blackwell Publishing, 2006, vol.~74, No~6, p.~265-276. ISSN~0301-4681.
|
