Analysis of tissues of vertebrates and earthworms treated by
Bromadiolone

D 2006

Analysis of tissues of vertebrates and earthworms treated by Bromadiolone

ADAM, Vojtěch, Miroslava BEKLOVÁ, René KIZEK, Soňa KŘÍŽKOVÁ, Radka MIKELOVÁ et. al.

Basic information

Original name

Analysis of tissues of vertebrates and earthworms treated by Bromadiolone

Name in Czech

Analýza tkání z hrabošů a žížal živených bromadiolonem

Authors

ADAM, Vojtěch (203 Czech Republic), Miroslava BEKLOVÁ (203 Czech Republic), René KIZEK (203 Czech Republic), Soňa KŘÍŽKOVÁ (203 Czech Republic), Radka MIKELOVÁ (203 Czech Republic, guarantor), J. PIKULA (203 Czech Republic), Michal SVOBODA (203 Czech Republic) and Veronika ŠUPLKOVÁ (203 Czech Republic)

Edition

prvn. Hamburg, 34th International Symposium on Environmental Analytical Chemistry, ISEAC 34, p. A29-A29, 405 pp. 2006

Publisher

International Association of Environmental Analytical Chemistry2006

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10405 Electrochemistry

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Science

Keywords in English

Electrochemistry; Bromadiolone; Lanirat; HPLC-ED; glassy carbon electrode; cyclic voltammetry;

Abstract

ORIG CZ

V originále

Bromadiolone (3-(3-(4'-brom-1,1'-bifenyl-4-yl)-3-hydroxy-1-fenylpropyl)-4-hydroxy-coumarine) is commonly used rodenticide, which is commercially available as Lanirat. It acts as anticoagulant in very low concentration and its effect is cumulative. Its residues in nature can be cause of death of both house and wild animals and not only in direct, but in consequent consuming. The main organ damaged during deposition and metabolizing of these substance is liver. In our work we developed a new way of bromadiolone detection based on use of high-performance liquid chromatography with electrochemical detection (HPLC-ED). Our method is quick, sensitive and allows us to determine a trace amount of bromadiolone in biological matrix. Moreover we are able to monitor the incidence of using of bromadiolone on non target fauna. Our analysis by high-performance liquid chromatography was carried out on CoulochemIII analyser (ESA, Inc.). Hydrogenpaladium electrode, auxiliary carbon electrode and working glassy carbon electrode were placed in electrochemical cell of volume of 50 ľl referent. We used acetate buffer (pH = 4.0):acetonitrile in ratio of 40/60 (v/v) as the most suitable mobile phase for determination of the compound of interest. Sample (5 ľl) was injected. Prior to analysis in flow system we have studied the basic electrochemical behaviour of bromadiolone by cyclic voltammetry on Autolab analyser (EcoChemie, Netherlands) connected with VA-Stand 663 (Metrohm, Schwitzerland). On the base of experimental results from cyclic voltammetry we designed optimal conditions for flow analysis of bromadiolone. From relation of signal height on applied potential we construed a hydrodynamical voltammogram. On its base we choose an optimal potential applied on electrodes (836 mV). Flow rate of mobile phase and the content of methanol influenced the electrochemical response significantly. We found out that our method is sensitive and allows us to determine tens of nanograms of bromadiolone per ml. We utilized this optimized method to determine the amount of bromadiolone in real samples of tissues of animal which eaten bromadiolone in different forms. Earthworms (Eisenia fetida), were kept in presence of five granules of rodenticide Lanirat G in five litres of artificial soil (88 ěg/l). These earthworms were used as a feed for field mice (Microtus arvalis). Other group of field mice was fed by defined amount of bromadiolone. Earthworms kept without rodenticide and field mice fed with non-treated earthworms or fed by usual feed for rodents served as a control. We analysed the content of bromadiolone in earthworms and in liver of these field mice. Then we applied this method to determine the bromadiolone content in liver and stomach content of three hares (Lepus europaeus), which were found death in area where bromadiolone was applied. We found out that one them died by bromadiolone.

In Czech

Analýza tkání z hrabošů a žížal živených bromadiolonem

Links

GP525/04/P132, research and development project

Name: Studium obranných mechanismů rostlin při stresu způsobeném těžkými kovy

MSM0021622412, plan (intention)

Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)

Citovat

ADAM, Vojtěch, Miroslava BEKLOVÁ, René KIZEK, Soňa KŘÍŽKOVÁ, Radka MIKELOVÁ, J. PIKULA, Michal SVOBODA and Veronika ŠUPLKOVÁ. Analysis of tissues of vertebrates and earthworms treated by Bromadiolone. In 34th International Symposium on Environmental Analytical Chemistry, ISEAC 34. prvn. Hamburg: International Association of Environmental Analytical Chemistry2006, 2006, p. A29-A29, 405 pp.

@inproceedings{638524,
   author = {Adam, Vojtěch and Beklová, Miroslava and Kizek, René and Křížková, Soňa and Mikelová, Radka and Pikula, J. and Svoboda, Michal and Šuplková, Veronika},
   address = {Hamburg},
   booktitle = {34th International Symposium on Environmental Analytical Chemistry, ISEAC 34},
   edition = {prvn},
   keywords = {Electrochemistry; Bromadiolone; Lanirat; HPLC-ED; glassy carbon electrode; cyclic voltammetry;},
   language = {eng},
   location = {Hamburg},
   pages = {A29-A29},
   publisher = {International Association of Environmental Analytical Chemistry2006},
   title = {Analysis of tissues of vertebrates and earthworms treated by Bromadiolone},
   year = {2006}
}

TY  - JOUR
ID  - 638524
AU  - Adam, Vojtěch - Beklová, Miroslava - Kizek, René - Křížková, Soňa - Mikelová, Radka - Pikula, J. - Svoboda, Michal - Šuplková, Veronika
PY  - 2006
TI  - Analysis of tissues of vertebrates and earthworms treated by Bromadiolone
PB  - International Association of Environmental Analytical Chemistry2006
CY  - Hamburg
KW  - Electrochemistry
KW  - Bromadiolone
KW  - Lanirat
KW  - HPLC-ED
KW  - glassy carbon electrode
KW  - cyclic voltammetry;
N2  - Bromadiolone (3-(3-(4'-brom-1,1'-bifenyl-4-yl)-3-hydroxy-1-fenylpropyl)-4-hydroxy-coumarine) is commonly used rodenticide, which is commercially available as Lanirat. It acts as anticoagulant in very low concentration and its effect is cumulative. Its residues in nature can be cause of death of both house and wild animals and not only in direct, but in consequent consuming. The main organ damaged during deposition and metabolizing of these substance is liver. In our work we developed a new way of bromadiolone detection based on use of high-performance liquid chromatography with electrochemical detection (HPLC-ED). Our method is quick, sensitive and allows us to determine a trace amount of bromadiolone in biological matrix. Moreover we are able to monitor the incidence of using of bromadiolone on non target fauna. Our analysis by high-performance liquid chromatography was carried out on CoulochemIII analyser (ESA, Inc.). Hydrogenpaladium electrode, auxiliary carbon electrode and working glassy carbon electrode were placed in electrochemical cell of volume of 50 ľl referent. We used acetate buffer (pH = 4.0):acetonitrile in ratio of 40/60 (v/v) as the most suitable mobile phase for determination of the compound of interest. Sample (5 ľl) was injected. Prior to analysis in flow system we have studied the basic electrochemical behaviour of bromadiolone by cyclic voltammetry on Autolab analyser (EcoChemie, Netherlands) connected with VA-Stand 663 (Metrohm, Schwitzerland). On the base of experimental results from cyclic voltammetry we designed optimal conditions for flow analysis of bromadiolone. From relation of signal height on applied potential we construed a hydrodynamical voltammogram. On its base we choose an optimal potential applied on electrodes (836 mV). Flow rate of mobile phase and the content of methanol influenced the electrochemical response significantly. We found out that our method is sensitive and allows us to determine tens of nanograms of bromadiolone per ml. We utilized this optimized method to determine the amount of bromadiolone in real samples of tissues of animal which eaten bromadiolone in different forms. Earthworms (Eisenia fetida), were kept in presence of five granules of rodenticide Lanirat G in five litres of artificial soil (88 ěg/l). These earthworms were used as a feed for field mice (Microtus arvalis). Other group of field mice was fed by defined amount of bromadiolone. Earthworms kept without rodenticide and field mice fed with non-treated earthworms or fed by usual feed for rodents served as a control. We analysed the content of bromadiolone in earthworms and in liver of these field mice. Then we applied this method to determine the bromadiolone content in liver and stomach content of three hares (Lepus europaeus), which were found death in area where bromadiolone was applied. We found out that one them died by bromadiolone.
ER  -

ADAM, Vojtěch, Miroslava BEKLOVÁ, René KIZEK, Soňa KŘÍŽKOVÁ, Radka MIKELOVÁ, J. PIKULA, Michal SVOBODA and Veronika ŠUPLKOVÁ. Analysis of tissues of vertebrates and earthworms treated by Bromadiolone. In \textit{34th International Symposium on Environmental Analytical Chemistry, ISEAC 34}. prvn. Hamburg: International Association of Environmental Analytical Chemistry2006, 2006, p.~A29-A29, 405 pp.

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