BARTÁK, Miloš, Hana VRÁBLÍKOVÁ, Jana ŠTEPIGOVÁ, Josef HÁJEK, Kristýna VEČEŘOVÁ, Jaroslav LANG and Peter VÁCZI. Antioxidative mechanisms in a lichen Lasallia pustulata exposed to light and dehydration stress. s (Antioxidative mechanisms in a lichen Lasallia pustulata exposed to light and dehydration stress.). In FESPB 2006 Congress: Book of Abstracts. Lyon (2006): University Lyon, 2006, p. 126-126.
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Basic information
Original name Antioxidative mechanisms in a lichen Lasallia pustulata exposed to light and dehydration stress. s
Authors BARTÁK, Miloš, Hana VRÁBLÍKOVÁ, Jana ŠTEPIGOVÁ, Josef HÁJEK, Kristýna VEČEŘOVÁ, Jaroslav LANG and Peter VÁCZI.
Edition Lyon (2006), FESPB 2006 Congress: Book of Abstracts, p. 126-126, 1 pp. 2006.
Publisher University Lyon
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 10600 1.6 Biological sciences
Country of publisher France
Confidentiality degree is not subject to a state or trade secret
WWW Congress website
Organization unit Faculty of Science
Keywords in English glutathione, zeaxanhtin, DEPS, photosynthesis, chlorophyll fluorescence
Changed by Changed by: prof. Ing. Miloš Barták, CSc., učo 57. Changed: 23/7/2006 14:32.
Abstract
In photosynthetizing partners of lichens, majority of stresses produce reactive oxygen species (ROS). Negative effects of ROS are alleviated by antioxidants, e.g. zeaxanthin, glutathione, catalase, superoxidedismutase. In previous studies, we demonstrated that the capacity of zeaxanthin (Z) formation is sufficient to cope with a high light (HL) stress in fully hydrated lichen thalli. The aim of presented study is to evaluate the dynamics of antioxidants in algal partner of L. pustulata exposed to several HL and dehydration regimes. Treated by a short-term HL (typically 1800 micromol m-2 s-1), hydrated lichens showed photoinhibition of photosynthesis (decrease in Fv/Fm, Yield PSII, increase in NPQ) and activation of antioxidative mechanisms. Z increased and DEPS reached 0.5, total glutathione decreased to a half of control and was largely oxidized. The changes were reversible, the above parameters reached pre-treated values within 10-24 h relaxation in dark. Dehydration at dark and LL (300 micromol m-2 s-1) led to the same DEPS in dry state reached after 5 h. However, DEPS increased more rapidly in LL than in dark during the early dehydration (water potential 0 to -10 MPa).
Abstract (in Czech)
Většina stresových stavů v rostlinné buňce vytváří reaktivní formy kyslíku(ROS), jejichž negativní účinky jsou zmírňovány pomocí antioxidantů: zeaxanthin, glutathione, catalase, superoxidedismutase. V předkládaném příspěvku uvádíme čadovou dynamiku antioxidantů v lišejníkových symbiotických řasách ve stélkách lišejníku Lasallia pustulata vystavených různým ovlivněním vysokými hodnotami záření a stresu vysycháním. Krátkodobá fotoinhibice byla navozena 1800 micromol m-2 s-1 u plně hydrovaných stélek. během fotoinhibice a následného zotavení byly sledovány fotosyntetické parametry Fv/Fm, Yield PS II, NPQ a dynamika antioxidantů: DEPS, množství a redoxní stav glutationu.
Links
GA522/03/0754, research and development projectName: Fotoinhibice a fotoochrana u lišejníků v extrémních podmínkách
Investor: Czech Science Foundation, Photoinhibition and photoprotection in lichens under extreme conditions
GA522/06/0979, research and development projectName: Mechanismy odolnosti rostlin proti ROS indukovaným ve fotosyntetickém aparátu během fotoinhibičního stresu
Investor: Czech Science Foundation, Mechanism of plant resistance against ROS induced in photosynthetic apparatus by a photoinhibitory stress
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