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@article{694386, author = {Krátká, J. and Pekárová, B. and Zemanová, A. and Kutíková, M.}, article_location = {Praha}, article_number = {1}, keywords = {Fusarium culmorum; winter wheat; polyclonal and monoclonal antibodies; PTA-ELISA; imunoprinting; dot blot; imunofluorescent microscopy}, issn = {1212-2580}, journal = {Ochrana rostlin}, title = {Utilization of immunochemical methods for the detection of Fusarium culmorum in winter wheat}, volume = {36}, year = {2000} }
TY - JOUR ID - 694386 AU - Krátká, J. - Pekárová, B. - Zemanová, A. - Kutíková, M. PY - 2000 TI - Utilization of immunochemical methods for the detection of Fusarium culmorum in winter wheat JF - Ochrana rostlin VL - 36 IS - 1 SP - 1-6 EP - 1-6 PB - Ústav zemědělských a potravinářských inf SN - 12122580 KW - Fusarium culmorum KW - winter wheat KW - polyclonal and monoclonal antibodies KW - PTA-ELISA KW - imunoprinting KW - dot blot KW - imunofluorescent microscopy N2 - Two monoclonal antibodies and one polyclonal antibody were prepared and use for the detection of Fusarium culmorum in winter wheat. In addition, Fusarium oxysporum, F. graminearum, F. solani, F. avenaceum, F. tricinctum, and F. moniliforme were used in tests. The antibodies were genus specific. Monoclonal antibodies did not cross-react with other fungal pathogens of winter wheat (Gaeumannomyces graminis, Psudocercosporella herpotrichoides, Rhizoctonia solani). The pathogen was detected in vitro, in hydrated and artificially inoculated kernels and in seedlings, by PTA-ELISA, immunoprinting, dot blot and immunofluorescent microscopy. Dot blot and immunofluorescence microscopy were the most sensitive method for recognising FCu in vitro and in vivo. Specificity and sensitivity of monoclonal and polyclonal antibodies were similar. ER -
KRÁTKÁ, J., B. PEKÁROVÁ, A. ZEMANOVÁ a M. KUTÍKOVÁ. Utilization of immunochemical methods for the detection of Fusarium culmorum in winter wheat. \textit{Ochrana rostlin}. Praha: Ústav zemědělských a potravinářských inf, roč.~36, č.~1, s.~1-6. ISSN~1212-2580. 2000.
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