Characterization of bacteriophage integrase gene families in
Staphylococcus aureus phages of the International Typing Set

D 2006

Characterization of bacteriophage integrase gene families in Staphylococcus aureus phages of the International Typing Set

PANTŮČEK, Roman, Vladislava RŮŽIČKOVÁ, Martina PETERKOVÁ, Petr PETRÁŠ, Jiří DOŠKAŘ et. al.

Basic information

Original name

Characterization of bacteriophage integrase gene families in Staphylococcus aureus phages of the International Typing Set

Name in Czech

Charakterizace genů pro bakteriofágové integrázy u fágů mezinárodní řady pro Staphylococcus aureus

Authors

PANTŮČEK, Roman (203 Czech Republic, guarantor), Vladislava RŮŽIČKOVÁ (203 Czech Republic), Martina PETERKOVÁ (203 Czech Republic), Petr PETRÁŠ (203 Czech Republic) and Jiří DOŠKAŘ (203 Czech Republic)

Edition

Abstract Book. Maastricht, the Netherlands, 12th International Symposium on Staphylococci & Staphylococcal Infections, p. 103-103, 2006

Publisher

Maastricht Exhibition & Congres Centre

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

Genetics and molecular biology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

RIV identification code

RIV/00216224:14310/06:00021700

Organization unit

Faculty of Science

Keywords in English

Staphylococcus aureus; medical microbiology; molecular diagnostics

Abstract

ORIG CZ

V originále

Temperate phages of family Siphoviridae play an important role in biology of S. aureus strains, usually changing their phenotype as a result of lysogenic conversion associated with some virulence factors. The prophage content of S. aureus strains can be determined by the multiplex PCR assay targeting structural genes of head-tail modules described previously (Pantůček et al., Arch. Virol. 2004; 149:1689). However, this approach did not occasionally cover defective prophages. Therefore, we focused on development, validation and application of a PCR-based molecular assay for identification and classification of S. aureus phages based on the sequences of their integrase genes. The integrase genes were sequenced in 23 phages of the International Typing Set by using primers derived from a conserved regulatory sequence between the integrase and excisionase genes followed by primer walking. Additional integrase gene sequences were retrieved from the GenBank database. For designation of bacteriophage integrase gene families the updated classification scheme was used which denotes the previously sequenced phage and S. aureus genomes. In this work the analyzed S. aureus phages were classified on the basis of integrase gene homology into 8 families which dictates at the same time insertion sites on the chromosome by a novel multiplex PCR strategy. Eight PCR primer pairs designed for the integrase sequences Sa1 (phiETA), Sa2 (phiPVL), Sa3 (phiN315), Sa4 (phiSa4-MSSA476), Sa5 (phi11), Sa6 (phiL54a), Sa7 (phi96) and Sa8 (phi77) shown to be very effective and capable in a single assay to identify phage integrase genes present in bacterial genomes in different combinations. This technique was verified on 100 clinical isolates and shown to be a useful tool for the identification of lysogenic variants ordinarily existing in S. aureus clones.

In Czech

neuvedeno

Links

MSM0021622415, plan (intention)

Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

Citovat

PANTŮČEK, Roman, Vladislava RŮŽIČKOVÁ, Martina PETERKOVÁ, Petr PETRÁŠ and Jiří DOŠKAŘ. Characterization of bacteriophage integrase gene families in Staphylococcus aureus phages of the International Typing Set. In 12th International Symposium on Staphylococci & Staphylococcal Infections. Abstract Book. Maastricht, the Netherlands: Maastricht Exhibition & Congres Centre, 2006, p. 103.

@inproceedings{695126,
   author = {Pantůček, Roman and Růžičková, Vladislava and Peterková, Martina and Petráš, Petr and Doškař, Jiří},
   address = {Maastricht, the Netherlands},
   booktitle = {12th International Symposium on Staphylococci & Staphylococcal Infections},
   edition = {Abstract Book},
   keywords = {Staphylococcus aureus; medical microbiology; molecular diagnostics},
   language = {eng},
   location = {Maastricht, the Netherlands},
   pages = {103-103},
   publisher = {Maastricht Exhibition & Congres Centre},
   title = {Characterization of bacteriophage integrase gene families in Staphylococcus aureus phages of the International Typing Set},
   url = {http://www.isssi2006.org/},
   year = {2006}
}

TY  - JOUR
ID  - 695126
AU  - Pantůček, Roman - Růžičková, Vladislava - Peterková, Martina - Petráš, Petr - Doškař, Jiří
PY  - 2006
TI  - Characterization of bacteriophage integrase gene families in Staphylococcus aureus phages of the International Typing Set
PB  - Maastricht Exhibition & Congres Centre
CY  - Maastricht, the Netherlands
KW  - Staphylococcus aureus
KW  - medical microbiology
KW  - molecular diagnostics
UR  - http://www.isssi2006.org/
N2  - Temperate phages of family Siphoviridae play an important role in biology of S. aureus strains, usually changing their phenotype as a result of lysogenic conversion associated with some virulence factors. The prophage content of S. aureus strains can be determined by the multiplex PCR assay targeting structural genes of head-tail modules described previously (Pantůček et al., Arch. Virol. 2004; 149:1689). However, this approach did not occasionally cover defective prophages. Therefore, we focused on development, validation and application of a PCR-based molecular assay for identification and classification of S. aureus phages based on the sequences of their integrase genes. The integrase genes were sequenced in 23 phages of the International Typing Set by using primers derived from a conserved regulatory sequence between the integrase and excisionase genes followed by primer walking. Additional integrase gene sequences were retrieved from the GenBank database. For designation of bacteriophage integrase gene families the updated classification scheme was used which denotes the previously sequenced phage and S. aureus genomes. In this work the analyzed S. aureus phages were classified on the basis of integrase gene homology into 8 families which dictates at the same time insertion sites on the chromosome by a novel multiplex PCR strategy. Eight PCR primer pairs designed for the integrase sequences Sa1 (phiETA), Sa2 (phiPVL), Sa3 (phiN315), Sa4 (phiSa4-MSSA476), Sa5 (phi11), Sa6 (phiL54a), Sa7 (phi96) and Sa8 (phi77) shown to be very effective and capable in a single assay to identify phage integrase genes present in bacterial genomes in different combinations. This technique was verified on 100 clinical isolates and shown to be a useful tool for the identification of lysogenic variants ordinarily existing in S. aureus clones.
ER  -

PANTŮČEK, Roman, Vladislava RŮŽIČKOVÁ, Martina PETERKOVÁ, Petr PETRÁŠ and Jiří DOŠKAŘ. Characterization of bacteriophage integrase gene families in $<$I$>$Staphylococcus aureus$<$/I$>$ phages of the International Typing Set. In \textit{12th International Symposium on Staphylococci \&{} Staphylococcal Infections}. Abstract Book. Maastricht, the Netherlands: Maastricht Exhibition \&{} Congres Centre, 2006, p.~103.

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