Detailed Information on Publication Record
2006
Proteomic characterization of the polyvalent staphylophage 812
DOŠKAŘ, Jiří, Luděk EYER, Roman PANTŮČEK, Vladislava RŮŽIČKOVÁ, Hana KONEČNÁ et. al.Basic information
Original name
Proteomic characterization of the polyvalent staphylophage 812
Name in Czech
Proteomic characterization of the polyvalent staphylophage 812
Authors
DOŠKAŘ, Jiří (203 Czech Republic, guarantor), Luděk EYER (203 Czech Republic), Roman PANTŮČEK (203 Czech Republic), Vladislava RŮŽIČKOVÁ (203 Czech Republic), Hana KONEČNÁ (203 Czech Republic), Zbyněk ZDRÁHAL (203 Czech Republic), Lenka HERNYCHOVÁ (203 Czech Republic) and Jan PREISLER (203 Czech Republic)
Edition
12th International Symposium on Staphylococci & Staphylococcal Infections, 2006
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
Genetics and molecular biology
Country of publisher
Netherlands
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
RIV identification code
RIV/00216224:14310/06:00015836
Organization unit
Faculty of Science
Keywords in English
Staphylococcus aureus; medical microbiology; molecular diagnostics; phage therapy; proteomics
Tags
International impact, Reviewed
Změněno: 8/4/2010 10:15, prof. Mgr. Jan Preisler, Ph.D.
V originále
Bacteriophage 812 is a polyvalent phage with very broad host range in the genus Staphylococcus, which makes it a suitable candidate for phage therapy of staphylococcal infections. The genomes of polyvalent staphylococcal phages exceed 125 kbp and include about 200 putative open reading frames. Our study was focused on characterization of the phage proteome, especially on identification of phage virion proteins. Combination of both one- and two-dimensional electrophoreses (1-DE and 2-DE) followed by peptide mass fingerprinting using MALDI-TOF mass spectrometry, led to the identification of 24 virion proteins of the phage 812. Twenty of them were not identified by proteome analysis of closely related staphylococcal phages K and G1 yet. Although the phage proteome is much simpler compared to that of cellular organisms, using 2-DE in addition to 1-DE significantly increased the number of identified proteins. Fifteen proteins were assigned unambiguously to the head-tail genome module; the remaining nine proteins are encoded by genes of the left or right arms of the phage genome. As expected, the most abundant proteins in the electrophoretic patterns are the major capsid protein, the major tail sheath protein and proteins identical to ORF 50 and ORF 95 of the phage K, although their function is only putative. Twenty new identified proteins set the basis for a detailed analysis of the phage virion structure and are a prerequisite for their efficient and safe application in phage therapy.
In Czech
neuvedeno
Links
| GA203/03/0515, research and development project |
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| MSM0021622415, plan (intention) |
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