Panton - Valentine leukocidin positive Staphylococcus aureus
strains isolated in the Czech Republic in 2004 - 2006

D 2006

Panton - Valentine leukocidin positive Staphylococcus aureus strains isolated in the Czech Republic in 2004 - 2006

PETRÁŠ, Petr, Ivana MACHOVÁ, Roman PANTŮČEK, Monika MAREJKOVÁ, Vladislava RŮŽIČKOVÁ et. al.

Basic information

Original name

Panton - Valentine leukocidin positive Staphylococcus aureus strains isolated in the Czech Republic in 2004 - 2006

Name in Czech

Panton - Valentine leukocidin positive Staphylococcus aureus strains isolated in the Czech Republic in 2004 - 2006

Authors

PETRÁŠ, Petr (203 Czech Republic), Ivana MACHOVÁ (203 Czech Republic), Roman PANTŮČEK (203 Czech Republic, guarantor), Monika MAREJKOVÁ (703 Slovakia), Vladislava RŮŽIČKOVÁ (203 Czech Republic) and Jiří DOŠKAŘ (203 Czech Republic)

Edition

Abstract Book. Maastricht, the Netherlands, 12th International Symposium on Staphylococci & Staphylococcal Infections, p. 225-225, 2006

Publisher

Maastricht Exhibition & Congres Centre

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

Genetics and molecular biology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

RIV identification code

RIV/00216224:14310/06:00017153

Organization unit

Faculty of Science

Keywords in English

Staphylococcus aureus; medical microbiology; molecular diagnostics; PVL

Abstract

ORIG CZ

V originále

Phenotypic and genotypic characteristics of 59 PVL positive strains isolated from clinical specimens in the Czech Republic are described. Methods: The RPLA method was used for detection of superantigens. To confirm toxigenicity PCR screening for the sea-sej, tst, eta and etb genes was carried out. Resistance to 12 antibiotics was tested by the disk diffusion method. Phage typing was performed by the standard method using the Int. Set of Phages. Carriage of the lukS-PV and lukF-PV genes for PVL and mecA gene was tested by a multiplex PCR method. The MRSA PVL+ strains were characterized by SmaI macrorestriction analysis resolved by PFGE, spa typing, SCCmec typing by multiplex PCR, prophage typing and preliminary matching of prophage types with PVL production. Results: The presence of the lukS-PV and lukF-PV genes was detected in 59 (6.6%) of 889 S. aureus strains isolated mainly from lesions, wound infection, skin and soft tissue abscesses. Production of enterotoxins A-D was found in 12 (20%) of the 59 PVL+ strains, two strains also produced TSST-1 and one strain was a producer of exfoliative toxin A. Only six strains were MRSA and were classified into SCCmec type IV, ordinarily found in community acquired MRSA strains. Genotypic properties of PVL+ MRSA strains were correlated with the known European MRSA types. Conclusions: The Czech Ref. Laboratory for Staphylococci has been paying attention to PVL+ S. aureus strains since 2004. By March 31, 2006, we found 59 (7.8%) of 889 isolates, mainly from lesions, to carry genes for PVL. Most (78%) PVL+ strains were phage typeable and were predominantly classified into phage group II (53%). Six MRSA PVL+ isolates carrying SCCmec type IV yielded five distinct and unrelated SmaI PFGE patterns and were of unrelated spa types 003, 008, 186, 311 and also 044 lukS-lukF positive CA-MRSA, widely disseminated in Europe.

In Czech

neuvedeno

Links

MSM0021622415, plan (intention)

Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

Citovat

PETRÁŠ, Petr, Ivana MACHOVÁ, Roman PANTŮČEK, Monika MAREJKOVÁ, Vladislava RŮŽIČKOVÁ and Jiří DOŠKAŘ. Panton - Valentine leukocidin positive Staphylococcus aureus strains isolated in the Czech Republic in 2004 - 2006. In 12th International Symposium on Staphylococci & Staphylococcal Infections. Abstract Book. Maastricht, the Netherlands: Maastricht Exhibition & Congres Centre, 2006, p. 225.

@inproceedings{695131,
   author = {Petráš, Petr and Machová, Ivana and Pantůček, Roman and Marejková, Monika and Růžičková, Vladislava and Doškař, Jiří},
   address = {Maastricht, the Netherlands},
   booktitle = {12th International Symposium on Staphylococci & Staphylococcal Infections},
   edition = {Abstract Book},
   keywords = {Staphylococcus aureus; medical microbiology; molecular diagnostics; PVL},
   language = {eng},
   location = {Maastricht, the Netherlands},
   pages = {225-225},
   publisher = {Maastricht Exhibition & Congres Centre},
   title = {Panton - Valentine leukocidin positive Staphylococcus aureus strains isolated in the Czech Republic in 2004 - 2006},
   url = {http://www.isssi2006.org/},
   year = {2006}
}

TY  - JOUR
ID  - 695131
AU  - Petráš, Petr - Machová, Ivana - Pantůček, Roman - Marejková, Monika - Růžičková, Vladislava - Doškař, Jiří
PY  - 2006
TI  - Panton - Valentine leukocidin positive Staphylococcus aureus strains isolated in the Czech Republic in 2004 - 2006
PB  - Maastricht Exhibition & Congres Centre
CY  - Maastricht, the Netherlands
KW  - Staphylococcus aureus
KW  - medical microbiology
KW  - molecular diagnostics
KW  - PVL
UR  - http://www.isssi2006.org/
N2  - Phenotypic and genotypic characteristics of 59 PVL positive strains isolated from clinical specimens in the Czech Republic are described. Methods: The RPLA method was used for detection of superantigens. To confirm toxigenicity PCR screening for the sea-sej, tst, eta and etb genes was carried out. Resistance to 12 antibiotics was tested by the disk diffusion method. Phage typing was performed by the standard method using the Int. Set of Phages. Carriage of the lukS-PV and lukF-PV genes for PVL and mecA gene was tested by a multiplex PCR method. The MRSA PVL+ strains were characterized by SmaI macrorestriction analysis resolved by PFGE, spa typing, SCCmec typing by multiplex PCR, prophage typing and preliminary matching of prophage types with PVL production. Results: The presence of the lukS-PV and lukF-PV genes was detected in 59 (6.6%) of 889 S. aureus strains isolated mainly from lesions, wound infection, skin and soft tissue abscesses. Production of enterotoxins A-D was found in 12 (20%) of the 59 PVL+ strains, two strains also produced TSST-1 and one strain was a producer of exfoliative toxin A. Only six strains were MRSA and were classified into SCCmec type IV, ordinarily found in community acquired MRSA strains. Genotypic properties of PVL+ MRSA strains were correlated with the known European MRSA types. Conclusions: The Czech Ref. Laboratory for Staphylococci has been paying attention to PVL+ S. aureus strains since 2004. By March 31, 2006, we found 59 (7.8%) of 889 isolates, mainly from lesions, to carry genes for PVL. Most (78%) PVL+ strains were phage typeable and were predominantly classified into phage group II (53%). Six MRSA PVL+ isolates carrying SCCmec type IV yielded five distinct and unrelated SmaI PFGE patterns and were of unrelated spa types 003, 008, 186, 311 and also 044 lukS-lukF positive CA-MRSA, widely disseminated in Europe.
ER  -

PETRÁŠ, Petr, Ivana MACHOVÁ, Roman PANTŮČEK, Monika MAREJKOVÁ, Vladislava RŮŽIČKOVÁ and Jiří DOŠKAŘ. Panton - Valentine leukocidin positive $<$I$>$Staphylococcus aureus$<$/I$>$ strains isolated in the Czech Republic in 2004 - 2006. In \textit{12th International Symposium on Staphylococci \&{} Staphylococcal Infections}. Abstract Book. Maastricht, the Netherlands: Maastricht Exhibition \&{} Congres Centre, 2006, p.~225.

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