MIKEŠ, Vladimír, Jan LOCHMAN and Tomáš KAŠPAROVSKÝ. Ergosterol is a signal molecule that leads to the expression of defence-related genes in tobacco. In Book of abstract of XX. Congress of IUBMB. 2006. ISSN 0037-1017.
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Basic information
Original name Ergosterol is a signal molecule that leads to the expression of defence-related genes in tobacco
Name in Czech Ergosterol je signální molekula vedoucí k expresi obranných genů u tabáku
Authors MIKEŠ, Vladimír, Jan LOCHMAN and Tomáš KAŠPAROVSKÝ.
Edition Book of abstract of XX. Congress of IUBMB, 2006.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 10600 1.6 Biological sciences
Country of publisher Japan
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 0.046
Organization unit Faculty of Science
ISSN 0037-1017
Keywords in English ergosterol plant defence
Tags ergosterol plant defence
Tags International impact
Changed by Changed by: prof. Mgr. Tomáš Kašparovský, Ph.D., učo 10078. Changed: 28/4/2011 20:02.
Abstract
Ergosterol is a principal compound of the fungal plasma membrane, which has never been found in plants. Previous studies have proved that ergosterol is able to trigger a defence reaction even in nano-molar concentration in tobacco and tomato cells. The reaction was characterized by production of active oxygen species (AOS), alkalinization of medium, changes in ion fluxes and production of phytoalexins (1,2) Some steps of the signalling cascade were also elucidated when the important role of phospholipase A2 together with protein kinase C and calcium were demonstrated (2,3). In this paper, the expression of some defence-related genes after the ergosterol treatment of tobacco Nicotiana tabacum plants is reported. The production of caposidiol was followed by HPLC. The gene expression of pathogenesis related proteins of families PR1, PR3, PR5 and proteinase inhibitors of class I and II together with enzymes participating in the defence response, such as phenylalanine ammonia lyase and sesquiterpene cyclase, were monitored by RT-qPCR. In addition, the concentrations of salicylic acid, an important signalling molecule, increased in time due to the enzyme activation. On the other hand, ergosterol did not provoke tissue necrosis and the possible cross-talk between the signalling pathways of salicylate and jasmonate was observed. Collected data shows that ergosterol is able to activate the expression of a number of defence genes, stimulate the synthesis of phytoalexins and could increase resistance against pathogens.
Abstract (in Czech)
Ergosterol je signální molekula vedoucí k expresi obranných genů u tabáku
Links
GA522/06/0156, research and development projectName: Vztah mezi strukturou a funkcí elicitoru Phytophthora spp.
Investor: Czech Science Foundation, Structure-function relationship of elicitors from Phytophthora spp.
MSM0021622413, plan (intention)Name: Proteiny v metabolismu a při interakci organismů s prostředím
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment
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