HAVLIŠ, Jan, Marek ŠEBELA, Tatiana ŠTOSOVÁ, Natalie WIELSCH, Henrik THOMAS, Zbyněk ZDRÁHAL and Andrej SHEVCHENKO. Thermostable trypsin conjugates for high-throughput proteomics: synthesis and performance evaluation. Proteomics. Weinheim: Wiley-VCH, 2006, vol. 10, No 6, p. 2959-2963, 4 pp. ISSN 1615-9853. |
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@article{695986, author = {Havliš, Jan and Šebela, Marek and Štosová, Tatiana and Wielsch, Natalie and Thomas, Henrik and Zdráhal, Zbyněk and Shevchenko, Andrej}, article_location = {Weinheim}, article_number = {6}, keywords = {Bovine trypsin; Conjugate; In-gel digestion; Oligosaccharide; PMF}, language = {eng}, issn = {1615-9853}, journal = {Proteomics}, title = {Thermostable trypsin conjugates for high-throughput proteomics: synthesis and performance evaluation}, volume = {10}, year = {2006} }
TY - JOUR ID - 695986 AU - Havliš, Jan - Šebela, Marek - Štosová, Tatiana - Wielsch, Natalie - Thomas, Henrik - Zdráhal, Zbyněk - Shevchenko, Andrej PY - 2006 TI - Thermostable trypsin conjugates for high-throughput proteomics: synthesis and performance evaluation JF - Proteomics VL - 10 IS - 6 SP - 2959-2963 EP - 2959-2963 PB - Wiley-VCH SN - 16159853 KW - Bovine trypsin KW - Conjugate KW - In-gel digestion KW - Oligosaccharide KW - PMF N2 - Conjugating bovine trypsin with oligosaccharides maltotriose, raffinose and stachyose increased its thermostability and suppressed autolysis, without affecting its cleavage specificity. These conjugates accelerated the digestion of protein substrates both in solution and in gel, compared to commonly used unmodified and methylated trypsins. ER -
HAVLIŠ, Jan, Marek ŠEBELA, Tatiana ŠTOSOVÁ, Natalie WIELSCH, Henrik THOMAS, Zbyněk ZDRÁHAL and Andrej SHEVCHENKO. Thermostable trypsin conjugates for high-throughput proteomics: synthesis and performance evaluation. \textit{Proteomics}. Weinheim: Wiley-VCH, 2006, vol.~10, No~6, p.~2959-2963, 4 pp. ISSN~1615-9853.
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