J 2006

Thermostable trypsin conjugates for high-throughput proteomics: synthesis and performance evaluation

HAVLIŠ, Jan, Marek ŠEBELA, Tatiana ŠTOSOVÁ, Natalie WIELSCH, Henrik THOMAS et. al.

Basic information

Original name

Thermostable trypsin conjugates for high-throughput proteomics: synthesis and performance evaluation

Name in Czech

Termostabilní trypsinové konjugáty pro proteomiku: synteza a zhodnocení aktivity

Authors

HAVLIŠ, Jan (203 Czech Republic, guarantor), Marek ŠEBELA (203 Czech Republic), Tatiana ŠTOSOVÁ (203 Czech Republic), Natalie WIELSCH (276 Germany), Henrik THOMAS (276 Germany), Zbyněk ZDRÁHAL (203 Czech Republic) and Andrej SHEVCHENKO (643 Russian Federation)

Edition

Proteomics, Weinheim, Wiley-VCH, 2006, 1615-9853

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 5.735

RIV identification code

RIV/00216224:14310/06:00017199

Organization unit

Faculty of Science

UT WoS

000238010400003

Keywords in English

Bovine trypsin; Conjugate; In-gel digestion; Oligosaccharide; PMF

Tags

Tags

Reviewed
Změněno: 26/6/2009 10:30, prof. RNDr. Zbyněk Zdráhal, Dr.

Abstract

ORIG CZ

V originále

Conjugating bovine trypsin with oligosaccharides maltotriose, raffinose and stachyose increased its thermostability and suppressed autolysis, without affecting its cleavage specificity. These conjugates accelerated the digestion of protein substrates both in solution and in gel, compared to commonly used unmodified and methylated trypsins.

In Czech

Konjugace trypsinu s oligosaccharidy (maltotriosa, rafinosa a stachyosa) zvyšuje jeho termostabilitu a potlačuje autolýzu bez ovlivnění jeho štěpící aktivity. Tyto konjugaty zvyšují digesci substrátů jak v roztoku, tak v gelu (ve srovnání s nemodifikovaným, resp. methylovaným trypsinem).

Links

LC06034, research and development project
Name: Regulace morfogeneze rostlinných buněk a orgánů
Investor: Ministry of Education, Youth and Sports of the CR, Regulation of morphogenesis of plant cells and organs
MSM0021622415, plan (intention)
Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations