| HAVLIŠ, Jan, Marek ŠEBELA, Tatiana ŠTOSOVÁ, Natalie WIELSCH, Henrik THOMAS, Zbyněk ZDRÁHAL and Andrej SHEVCHENKO. Thermostable trypsin conjugates for high-throughput proteomics: synthesis and performance evaluation. Proteomics. Weinheim: Wiley-VCH, 2006, vol. 10, No 6, p. 2959-2963, 4 pp. ISSN 1615-9853. |
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@article{695986,
author = {Havliš, Jan and Šebela, Marek and Štosová, Tatiana and Wielsch, Natalie and Thomas, Henrik and Zdráhal, Zbyněk and Shevchenko, Andrej},
article_location = {Weinheim},
article_number = {6},
keywords = {Bovine trypsin; Conjugate; In-gel digestion; Oligosaccharide; PMF},
language = {eng},
issn = {1615-9853},
journal = {Proteomics},
title = {Thermostable trypsin conjugates for high-throughput proteomics: synthesis and performance evaluation},
volume = {10},
year = {2006}
}
TY - JOUR
ID - 695986
AU - Havliš, Jan - Šebela, Marek - Štosová, Tatiana - Wielsch, Natalie - Thomas, Henrik - Zdráhal, Zbyněk - Shevchenko, Andrej
PY - 2006
TI - Thermostable trypsin conjugates for high-throughput proteomics: synthesis and performance evaluation
JF - Proteomics
VL - 10
IS - 6
SP - 2959-2963
EP - 2959-2963
PB - Wiley-VCH
SN - 16159853
KW - Bovine trypsin
KW - Conjugate
KW - In-gel digestion
KW - Oligosaccharide
KW - PMF
N2 - Conjugating bovine trypsin with oligosaccharides maltotriose, raffinose and stachyose increased its thermostability and suppressed autolysis, without affecting its cleavage specificity. These conjugates accelerated the digestion of protein substrates both in solution and in gel, compared to commonly used unmodified and methylated trypsins.
ER -
HAVLIŠ, Jan, Marek ŠEBELA, Tatiana ŠTOSOVÁ, Natalie WIELSCH, Henrik THOMAS, Zbyněk ZDRÁHAL and Andrej SHEVCHENKO. Thermostable trypsin conjugates for high-throughput proteomics: synthesis and performance evaluation. \textit{Proteomics}. Weinheim: Wiley-VCH, 2006, vol.~10, No~6, p.~2959-2963, 4 pp. ISSN~1615-9853.
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