Protein Modification Degree - An Indirect MS Quantitation Approach.
| HAVLIŠ, Jan, Zbyněk ZDRÁHAL and Ondrej ŠEDO. Protein Modification Degree - An Indirect MS Quantitation Approach. In Abstract Book 17th IMSC Prague. 2006. |
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| Basic information | |
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| Original name | Protein Modification Degree - An Indirect MS Quantitation Approach. |
| Name in Czech | Stanovení rozsahu modifikace - nepřímý přístup pomocí hmotnostní spektrometrie |
| Authors | HAVLIŠ, Jan (203 Czech Republic, guarantor), Zbyněk ZDRÁHAL (203 Czech Republic) and Ondrej ŠEDO (203 Czech Republic). |
| Edition | Abstract Book 17th IMSC Prague, 2006. |
| Other information | |
|---|---|
| Original language | English |
| Type of outcome | Conference abstract |
| Field of Study | 10600 1.6 Biological sciences |
| Country of publisher | Czech Republic |
| Confidentiality degree | is not subject to a state or trade secret |
| RIV identification code | RIV/00216224:14310/06:00017200 |
| Organization unit | Faculty of Science |
| Keywords in English | mass spectrometry; post-translational modification; modification degree |
| Tags | mass spectrometry, modification degree, post-translational modification |
| Changed by | Changed by: doc. Mgr. Jan Havliš, Dr., učo 743. Changed: 9/4/2010 13:36. |
| Abstract |
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| The method presented allows to quantify the protein modification degree (PMD) by means of determination of either level of modification or demodification. It is also shown, that there is no need for strictly controlled isotopic purity of H218O. Moreover, the importance of concentration correction (CC) is shown. The experiments were accompanied with statistical tests. |
| Abstract (in Czech) |
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| Prezentovaná metoda umožňuje stranovit rozsah modifikace proteinu; ať už přímo nebo jako míru demofikace. Rovněž bylo ukázáno, že požadavek na izotopovou čistotu H218O není striktní. Spolu s tím byl zdůrazněn význam koncentreční korekce. Experimenty byly doprovázeny statistickými testy. |
| Links | |
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| LC06034, research and development project | Name: Regulace morfogeneze rostlinných buněk a orgánů |
| Investor: Ministry of Education, Youth and Sports of the CR, Regulation of morphogenesis of plant cells and organs | |
| MSM0021622413, plan (intention) | Name: Proteiny v metabolismu a při interakci organismů s prostředím |
| Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment | |
| MSM0021622415, plan (intention) | Name: Molekulární podstata buněčných a tkáňových regulací |
| Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations | |
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