J 2006

Isolation and characterization of a novel semi-lethal Arabidopsis thaliana mutant of gene for pentatricopeptide (PPR) repeat-containing protein

KOCÁBEK, Tomáš; Jana ŘEPKOVÁ; Markéta DUDOVÁ; Klára HOYEROVÁ; Lukáš VRBA et. al.

Basic information

Original name

Isolation and characterization of a novel semi-lethal Arabidopsis thaliana mutant of gene for pentatricopeptide (PPR) repeat-containing protein

Name in Czech

Izolace a charakterizace nové semiletální mutace Arabidopsis thaliana v genu pro protein obsahující pentatricopeptide (PPR) motiv

Authors

KOCÁBEK, Tomáš; Jana ŘEPKOVÁ; Markéta DUDOVÁ; Klára HOYEROVÁ and Lukáš VRBA

Edition

Genetica, Nizozemí, Springer Netherlands, 2006, 0016-6707

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

Genetics and molecular biology

Country of publisher

Latvia

Confidentiality degree

is not subject to a state or trade secret

Impact factor

Impact factor: 1.492

RIV identification code

RIV/00216224:14310/06:00015893

Organization unit

Faculty of Science

UT WoS

000241139800034

Keywords in English

Arabidopsis thaliana - embryonic defect - mitochondria - pentatricopeptide repeat - semi-lethality - T-DNA mutagenesis
Changed: 23/11/2006 07:52, prof. RNDr. Jana Řepková, CSc.

Abstract

ORIG CZ

In the original language

A novel Arabidopsis thaliana mutant of one member of the PPR (pentatricopeptide repeat) gene family has been identified among T-DNA insertion lines. Tagging of the At1g53330 gene caused the appearance of a semi-lethal mutation with a complex phenotypic expression from embryo lethality associated with the abnormal pattern of cell division during globular to heart transition to fertile plants with just subtle phenotypic changes. The PPR protein At1g53330.1 was predicted to be targeted to mitochondria by TargetP and MitoProt programs. Complementation analysis comfirmed that phenotype is a result of a single T-DNA integration. A thorough functional analysis of this mutant aimed at finding a particular organelle target of At1g53330.1 protein will follow.

In Czech

Mezi T-DNA inzerčními liniemi Arabidopsis thaliana byl identifován mutant v genu z rodiny pentatricopeptide repeat (PPR). Inzerce v genu At153330 způsobila semiletální mutaci s komplexním fenotypovým projevem včetně embryonální letality. Protein PPR je transportován do mitochondrií. Komplementační analýza potvrdila, že mutace je způsobena jedním inzertem T-DNA.

Links

GD204/05/H505, research and development project
Name: Vývojová genetika rostlin
Investor: Czech Science Foundation, Developmental and Evolutionary Genetics of Plants
MSM 143100008, plan (intention)
Name: Genomy a jejich funkce
Investor: Ministry of Education, Youth and Sports of the CR, Genomes and their functions
MSM0021622415, plan (intention)
Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations