Detailed Information on Publication Record
2006
Chemotaxonomic Characterisation of Pseudomonads by MALDI-TOF MS
ŠEDO, Ondrej, Andrea VÁVROVÁ, Matej LEXA, Ludmila TVRZOVÁ, Zbyněk ZDRÁHAL et. al.Basic information
Original name
Chemotaxonomic Characterisation of Pseudomonads by MALDI-TOF MS
Name in Czech
Chemotaxonomická charakterizace pseudomonád pomocí MALDI-TOF MS
Authors
ŠEDO, Ondrej (203 Czech Republic), Andrea VÁVROVÁ (203 Czech Republic), Matej LEXA (703 Slovakia), Ludmila TVRZOVÁ (203 Czech Republic) and Zbyněk ZDRÁHAL (203 Czech Republic, guarantor)
Edition
17th International Mass Spectrometry Conference Abstract Book, 2006
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10600 1.6 Biological sciences
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
RIV identification code
RIV/00216224:14310/06:00017393
Organization unit
Faculty of Science
Keywords in English
MALDI; Proteins; Pseudomonads; Chemotaxonomy
Tags
Tags
International impact, Reviewed
Změněno: 9/4/2010 13:41, prof. RNDr. Zbyněk Zdráhal, Dr.
V originále
Pseudomonads are wide-spread bacteria known for their degradation abilities. Bacteria of the genus are ubiquitous in soil, water and many other natural habitats. Their main importance lies in modification of enviroment (geochemical cycles, biodegradation). Some members of the genus are important pathogens of plants, animals and human. The genus Pseudomonas sensu stricto comprises 160 validly described species and 8 subspecies, at present. Classification of environmental Pseudomonas isolates is often complicated due to heterogeneity and genetic variability of the species. Analysis of intact bacteria cells by Matrix-Assisted Laser Desorption Ionisation Time-of-Flight Mass Spectrometry (MALDI-TOF MS) has been shown to provide characteristic peptide/protein profiles which enable rapid identification of the microorganisms.1 In our work, sample preparation procedures were optimised for this group of Gram-negative bacteria including culture preparation and conditions of MALDI-MS analysis. MS data were processed with software developed at the Department of Informatics. The processing step involves three separate procedures: i) peak recognition and quantification, ii) calculation of inter-spectra similarity values based on selected metrics (shared peak count and its modifications, vector cosine and euclidian distance) and iii) clustering and classification based on the obtained similarity data. Results show our ability to distinguish subtle differences between the samples, well below the species level. MALDI MS has proven as a useful technique for fast taxonomic characterisation of pseudomonads. 1. C. Fenselau, P.A. Demirev, Mass Spectrom. Rev. 20, 157 (2001).
In Czech
Na základě MALDI-TOF hmotnostních spekter a charakterizovány různé kmeny rodu Pseudomonas.
Links
| LC06034, research and development project |
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| MSM0021622413, plan (intention) |
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| MSM0021622415, plan (intention) |
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