DNA Binding and Strand Annealing Properties of the Human BRCA2 DNA Binding Domain

KREJČÍ, Lumír. DNA Binding and Strand Annealing Properties of the Human BRCA2 DNA Binding Domain. Paris: Institute Curie, 2006.

Basic information

Original name

DNA Binding and Strand Annealing Properties of the Human BRCA2 DNA Binding Domain

Name in Czech

Charakterizace DNA vazebné domény proteinu BRCA2

Authors

KREJČÍ, Lumír

Edition

Paris, 2006

Publisher

Institute Curie

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Other information

Language

English

Type of outcome

Audiovizuální tvorba

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Organization unit

Faculty of Science

Keywords in English

Rekombinace; oprava; DNA

Tags

DNA, oprava, Rekombinace

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Abstract

V originále

Mutations in the BRCA2 (breast cancer susceptibility gene 2) gene give rise to a significant portion of familial breast cancers. BRCA2 associates with Rad51, a key member of the RAD52 group, through a series of conserved modules called BRC repeats. Rad51 has a recombinase activity that mediates the formation of DNA joints between the injured chromosome and an intact homologue during HR. In mediating HR reactions, Rad51 nucleates onto single-stranded (ss) DNA derived from lesion processing to form a highly ordered nucleoprotein filament structure, often referred to as the presynaptic filament. After locating a DNA homologue, the presynaptic filament catalyzes pairing with the homologue to form a nascent DNA joint called a D-loop, the length of which is extended by DNA strand exchange. Concurrently, DNA synthesis commences to replace the genetic information lost during the lesion processing step. Subsequent steps include resolution of recombination intermediates and ligation to complete the recombination/repair reaction.

In Czech

V příspěvku je uvedena biochemická charakterizace DNA vazebné domény lidského proteinu BRCA2. Jednak její schopnost vázat různe druhy DNA podle jejich sekundární struktury a katalyzovat nasedáni komplementárních řetězců DNA.

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Links

LC06030, research and development project	Name: Biomolekulární centrum Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular centre
ME 888, research and development project	Name: Srs2 protein a jeho multifunkční úloha při rekombinančních /opravných procesech Investor: Ministry of Education, Youth and Sports of the CR, Srs2 protein and its multi-functional role in rekombination/repair processes
MSM0021622413, plan (intention)	Name: Proteiny v metabolismu a při interakci organismů s prostředím Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment