FILLIPO, Joseph, Peter CHI, Lumír KREJČÍ and Patrick SUNG. Recombination Mediator and Rad51 Targeting Activities of a Human BRCA2 Polypeptide. J. Biol. Chem. 2006, vol. 17, No 281, p. 11649-11657, 8 pp. ISSN 0021-9258. |
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@article{699840, author = {Fillipo, Joseph and Chi, Peter and Krejčí, Lumír and Sung, Patrick}, article_number = {281}, keywords = {BRCA2-Rad51 interactions}, language = {eng}, issn = {0021-9258}, journal = {J. Biol. Chem.}, title = {Recombination Mediator and Rad51 Targeting Activities of a Human BRCA2 Polypeptide}, url = {http://www.jbc.org/cgi/content/abstract/281/17/11649}, volume = {17}, year = {2006} }
TY - JOUR ID - 699840 AU - Fillipo, Joseph - Chi, Peter - Krejčí, Lumír - Sung, Patrick PY - 2006 TI - Recombination Mediator and Rad51 Targeting Activities of a Human BRCA2 Polypeptide JF - J. Biol. Chem. VL - 17 IS - 281 SP - 11649-11657 EP - 11649-11657 SN - 00219258 KW - BRCA2-Rad51 interactions UR - http://www.jbc.org/cgi/content/abstract/281/17/11649 N2 - BRCA2 likely exerts its tumor suppressor function by enhancing the efficiency of the homology-directed repair of injured chromosomes. To help define the DNA repair role of BRCA2, we expressed and purified a polypeptide, BRC3/4-DBD, that harbors its BRC3 and BRC4 repeats and DNA binding domain. BRC3/4-DBD interacted with hRad51 and bound DNA with a distinct preference for single-stranded (ss) DNA. Importantly we demonstrated by biochemical means and electron microscopy that BRC3/4-DBD nucleates hRad51 onto ssDNA and acts as a recombination mediator in enabling hRad51 to utilize replication protein A-coated ssDNA as recombination substrate. These functions of BRC3/4-DBD required both the BRC repeats and the BRCA2 DNA binding domain. The results thus clarify the role of BRCA2 in Rad51-dependent DNA recombination and repair, and the experimental strategies described herein should be valuable for systematically deciphering this BRCA2 function. ER -
FILLIPO, Joseph, Peter CHI, Lumír KREJČÍ and Patrick SUNG. Recombination Mediator and Rad51 Targeting Activities of a Human BRCA2 Polypeptide. \textit{J. Biol. Chem.}. 2006, vol.~17, No~281, p.~11649-11657, 8 pp. ISSN~0021-9258.
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