Srs2 protein and its multi-functional role in recombination/repair processes

KREJČÍ, Lumír. Srs2 protein and its multi-functional role in recombination/repair processes. Hiedelberg: EMBO, 2006.

Basic information

• Original name: Srs2 protein and its multi-functional role in recombination/repair processes
• Name in Czech: Srs2 protein a jeho multi-funkční úlohy při rekombinaci a DNA opravě
• Authors: KREJČÍ, Lumír.
• Edition: Hiedelberg, 2006.
• Publisher: EMBO

Other information

• Original language: English
• Type of outcome: Audiovisual works
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Austria
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Organization unit: Faculty of Science
• Keywords in English: Srs2 protein; recombination; DNA repair
• Tags: DNA repair, recombination, Srs2 protein

Abstract

Homologous recombination (HR) plays a key role in the elimination of cytotoxic and genotoxic lesions induced by ionizing radiation, carcinogenic chemicals, and chemotherapeutic compounds. Furthermore, cells have to rely on homologous recombination to rescue stalled replication forks and hence prevent fork demise. If not processed properly, DSBs can lead to chromosomal aberrations and rearrangements, which then give rise to strong mutator and cancer phenotypes. Genetic analyses in the yeast (Saccharomyces cerevisiae) have revealed a central role of the Srs2 helicase in preventing untimely HR events and the formation of potentially deleterious DNA structures upon DNA replication stress. In contrast, Srs2 function appears to be important also for proper repair of DNA double-strand breaks by HR. In this work, we aim to dissect the multi-functional role of Srs2 in HR and other cellular processes using combination of biochemical, genetic and structural approaches.

Abstract (in Czech)

Homologous recombination (HR) plays a key role in the elimination of cytotoxic and genotoxic lesions induced by ionizing radiation, carcinogenic chemicals, and chemotherapeutic compounds. Furthermore, cells have to rely on homologous recombination to rescue stalled replication forks and hence prevent fork demise. If not processed properly, DSBs can lead to chromosomal aberrations and rearrangements, which then give rise to strong mutator and cancer phenotypes. Genetic analyses in the yeast (Saccharomyces cerevisiae) have revealed a central role of the Srs2 helicase in preventing untimely HR events and the formation of potentially deleterious DNA structures upon DNA replication stress. In contrast, Srs2 function appears to be important also for proper repair of DNA double-strand breaks by HR. In this work, we aim to dissect the multi-functional role of Srs2 in HR and other cellular processes using combination of biochemical, genetic and structural approaches.

Links

• LC06030, research and development project: Name: Biomolekulární centrum

Investor: Ministry of Education, Youth and Sports of the CR, Biomolecular centre

• ME 888, research and development project: Name: Srs2 protein a jeho multifunkční úloha při rekombinančních /opravných procesech

Investor: Ministry of Education, Youth and Sports of the CR, Srs2 protein and its multi-functional role in rekombination/repair processes

• MSM0021622413, plan (intention): Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment