| KUČERA, Igor. Interference of chlorate and chlorite with nitrate reduction in resting cells of Paracoccus denitrificans. Microbiology-SGM. Reading, Berks (Great Britain): Society for General Microbiology, 2006, roč. 152, č. 12, s. 3529-3534. ISSN 1350-0872. |
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@article{700507,
author = {Kučera, Igor},
article_location = {Reading, Berks (Great Britain)},
article_number = {12},
keywords = {nitrate respiration; nitrate reductase; chlorate; chlorite; enzyme inactivation},
language = {eng},
issn = {1350-0872},
journal = {Microbiology-SGM},
title = {Interference of chlorate and chlorite with nitrate reduction in resting cells of Paracoccus denitrificans},
volume = {152},
year = {2006}
}
TY - JOUR
ID - 700507
AU - Kučera, Igor
PY - 2006
TI - Interference of chlorate and chlorite with nitrate reduction in resting cells of Paracoccus denitrificans
JF - Microbiology-SGM
VL - 152
IS - 12
SP - 3529-3534
EP - 3529-3534
PB - Society for General Microbiology
SN - 13500872
KW - nitrate respiration
KW - nitrate reductase
KW - chlorate
KW - chlorite
KW - enzyme inactivation
N2 - When grown anaerobically on a succinate-nitrate medium (SN), Paracoccus denitrificans forms the membrane-bound, cytoplasmically-oriented, chlorate-reducing nitrate reductase Nar, while the periplasmic enzyme Nap is expressed during the aerobic growth on butyrate (BO). Preincubation of SN cells with chlorate produced a concentration-dependent decrease in nitrate utilization, which could be ascribed to Nar inactivation. Toluenisation rendered Nar less sensitive to chlorate, but more sensitive to chlorite. Nap enzyme of BO cells was inactivated by both chlorate and chlorite at concentrations that were at least two orders of magnitude lower than those shown to affect Nar. Partial purification of Nap resulted in an insensitivity to chlorate and a diminished sensitivity to chlorite. Azide was specific for SN cells in protecting nitrate reductase against chlorate attack, the protective effect of nitrate being more pronounced in BO cells. The results are discussed in terms of different metabolic activation of chlorine oxoanions in both types of cells and limited permeation of chlorite across the cell membrane.
ER -
KUČERA, Igor. Interference of chlorate and chlorite with nitrate reduction in resting cells of Paracoccus denitrificans. \textit{Microbiology-SGM}. Reading, Berks (Great Britain): Society for General Microbiology, 2006, roč.~152, č.~12, s.~3529-3534. ISSN~1350-0872.
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