Detailed Information on Publication Record
2006
Interference of chlorate and chlorite with nitrate reduction in resting cells of Paracoccus denitrificans
KUČERA, IgorBasic information
Original name
Interference of chlorate and chlorite with nitrate reduction in resting cells of Paracoccus denitrificans
Name in Czech
Ovlivnění redukce dusičnanu v nerostoucích buňkách Paracoccus denitrificans chlorečnanem a chloritanem
Authors
KUČERA, Igor (203 Czech Republic, guarantor)
Edition
Microbiology-SGM, Reading, Berks (Great Britain), Society for General Microbiology, 2006, 1350-0872
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 3.139
RIV identification code
RIV/00216224:14310/06:00017489
Organization unit
Faculty of Science
UT WoS
000243205800007
Keywords in English
nitrate respiration; nitrate reductase; chlorate; chlorite; enzyme inactivation
Tags
International impact, Reviewed
Změněno: 16/2/2007 07:07, prof. RNDr. Igor Kučera, DrSc.
V originále
When grown anaerobically on a succinate-nitrate medium (SN), Paracoccus denitrificans forms the membrane-bound, cytoplasmically-oriented, chlorate-reducing nitrate reductase Nar, while the periplasmic enzyme Nap is expressed during the aerobic growth on butyrate (BO). Preincubation of SN cells with chlorate produced a concentration-dependent decrease in nitrate utilization, which could be ascribed to Nar inactivation. Toluenisation rendered Nar less sensitive to chlorate, but more sensitive to chlorite. Nap enzyme of BO cells was inactivated by both chlorate and chlorite at concentrations that were at least two orders of magnitude lower than those shown to affect Nar. Partial purification of Nap resulted in an insensitivity to chlorate and a diminished sensitivity to chlorite. Azide was specific for SN cells in protecting nitrate reductase against chlorate attack, the protective effect of nitrate being more pronounced in BO cells. The results are discussed in terms of different metabolic activation of chlorine oxoanions in both types of cells and limited permeation of chlorite across the cell membrane.
In Czech
Při anaerobním růstu na mediu se sukcinátem a nitrátem (SN) vytváří Paracoccus denitrificans membránově vázanou nitrátreduktasu Nar, zatímco při aerobním růstu na butyrátu (BO) vzniká periplazmatický enzym Nap. Preinkubace SN buněk s chlorečnanem vedla k inaktivaci Nar. Toluenizace snižovala citlivost Nar k chlorečnanu a zvyšovala citlivost k chloritanu. Nap v BO buňkách byla inaktivována již o tři řády nižšími koncentracemi chlorečnanu i chloritanu. Částečně purifikovaná Nap byla necitlivá k chlorečnanu a méně citlivá k chloritanu. Nar před působením chlorečnanu specificky chránil azid, Nap dusičnan. Výsledky svědčí o rozdílné metabolické aktivaci oxoaniontů Cl v obou typech buněk a o omezené propustnosti buněčné membrány pro chloritan.
Links
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