REIFFOVÁ, Katarina, Jana PODOLONIČOVÁ, Lucia ONOFREJOVÁ, Jan PREISLER and Radomíra NEMCOVÁ. Thin-layer Chromatography and Matrix-assisted Laser Desorption/Ionization Mass Spectrometric Analysis of Oligosaccharides in Biological Samples. JPC-J. Planar Chromatogr.-Mod. TLC. 2007, vol. 20, No 1, p. 19-25. ISSN 0933-4173.
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Basic information
Original name Thin-layer Chromatography and Matrix-assisted Laser Desorption/Ionization Mass Spectrometric Analysis of Oligosaccharides in Biological Samples
Name in Czech Chromatografie na tenké vrstvě a hmotnostně spektrometrická analýza oligosacharidů v biologických vzorcích laserovou desorpcí/ionizací za účasti matrice
Authors REIFFOVÁ, Katarina (703 Slovakia), Jana PODOLONIČOVÁ (703 Slovakia), Lucia ONOFREJOVÁ (203 Czech Republic, belonging to the institution), Jan PREISLER (203 Czech Republic, guarantor, belonging to the institution) and Radomíra NEMCOVÁ (703 Slovakia).
Edition JPC-J. Planar Chromatogr.-Mod. TLC, 2007, 0933-4173.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10406 Analytical chemistry
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 0.683
RIV identification code RIV/00216224:14310/07:00025567
Organization unit Faculty of Science
UT WoS 000245524500005
Keywords in English TLC; MALDI MS; oligosaccharides
Tags MALDI MS, oligosaccharides, TLC
Changed by Changed by: Mgr. Eva Špillingová, učo 110713. Changed: 14/8/2017 13:03.
Abstract
TLC and MALDI MS of Oligosaccharides in Biological Samples. Thin-layer chromatography (TLC) and matrix-assisted laser desorption/ ionization mass spectrometry (MALDI MS) have been used for analysis of fructooligosaccharides (FOS), used as feed additives (prebiotics), in biological samples. The contents of different regions of a piglet’s intestine (jejunum, ileum, cecum, and colon) were analyzed. Raftilose, a commercially available dietetic product which contains fructooligosaccharides, was added into the feed of the weaning piglets. Complicated biological samples for TLC analysis were used with minimal pretreatment. Analysis was performed on glass-backed precoated silica gel layers impregnated with sodium acetate and developed with butanol–acetic acid–water, 2 + 2 + 1 (v/v), in a vertical glass twin-trough chamber with mobile phase vapor saturation. Visualization of FOS on chromatograms was achieved with a mixture of diphenylamine, aniline, and phosphoric acid in acetone as primary detection reagent. Blue–pink spots of FOS on chromatograms were also detected by reflectance densitometry at lambda = 370 nm. Optimum MALDI MS conditions for analysis of Raftilose standard were determined. By use of 10 mg mL–1 2,5-dihydroxybenzoic acid solution and addition of 1 mg mL–1 sodium acetate, a limit of detection (LOD) of 1.4 x 10–4% Raftilose was achieved; this corresponded to ~0.5 ng Raftilose deposited on the target. The effect of biological medium (intestine contents) on the MALDI spectra of Raftilose was checked. LODs of Raftilose in the presence of biological medium were determined from the spectra of samples taken from the ileum and jejunum spiked with 0.05 mg mL–1 Raftilose standard.
Abstract (in Czech)
TLC and MALDI MS oligosacharidů v biologických vzorcích
Links
MSM0021622415, plan (intention)Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
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