Adhesion of eukaryotic cell lines on the gold surface modified
with extracellular matrix proteins monitored by the
piezoelectric sensor

J 2007

Adhesion of eukaryotic cell lines on the gold surface modified with extracellular matrix proteins monitored by the piezoelectric sensor

FOHLEROVÁ, Zdenka, Petr SKLÁDAL and Jaroslav TURÁNEK

Basic information

Original name

Adhesion of eukaryotic cell lines on the gold surface modified with extracellular matrix proteins monitored by the piezoelectric sensor

Name in Czech

Adheze eukaryotickych buecnych linii na povrch zlata modifikovany extracelularnimi bunecnymi proteiny

Authors

FOHLEROVÁ, Zdenka (203 Czech Republic), Petr SKLÁDAL (203 Czech Republic, guarantor) and Jaroslav TURÁNEK (203 Czech Republic)

Edition

Biosensors & Bioelectronics, Oxford, Elsevier Advanced Technology, 2007, 0956-5663

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.061

RIV identification code

RIV/00216224:14310/07:00021741

Organization unit

Faculty of Science

UT WoS

000245541600009

Keywords in English

Piezoelectric biosensor; Reisstance; Resonant frequency; Laminin; Fibronectin; Vitronectin

Abstract

ORIG CZ

V originále

The piezoelectric sensor (quartz crystal microbalance, QCM) was used to monitor cell adhesion in real time. Two cell lines, rat epithelial cells (WB F344) and lung melanoma cells (B16F10) were used. The cells were adhered and grown on the gold surface of the sensor pre-coated with adsorbed layer of extracellular matrix proteins as vitronectin and laminin. The process of cell attachment and spreading on the gold surface was continuously monitored and displayed by changes of the resonant frequency and resistance values of the piezoelectric resonators. The initial phase of cell attachment and spreading induced a decrease of frequency and increase of resistance relating viscoelastic properties of the cell monolayer on the sensing surface. The steady-state of both shifts was achieved after a few hours. The presence and state of cells on the surface was confirmed by fluorescent microscopy. The obtained results demonstrate that the piezoelectric sensor is suitable for studies of the cell adhesion processes. Thus obtained cell-based biosensor has potential for identification and screening of biologically active drugs and other biomolecules affecting cellular shape and attachment.

In Czech

Pomoci piezoelektrickeho biosensoru byla studovana adheze bunecnych linii na povrch zlata modifikovany vhodnymi proteiny.

Links

MSM0021622413, plan (intention)

Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment

Citovat

FOHLEROVÁ, Zdenka, Petr SKLÁDAL and Jaroslav TURÁNEK. Adhesion of eukaryotic cell lines on the gold surface modified with extracellular matrix proteins monitored by the piezoelectric sensor. Biosensors & Bioelectronics. Oxford: Elsevier Advanced Technology, 2007, vol. 22, 9-10, p. 1896-1901. ISSN 0956-5663.

@article{701750,
   author = {Fohlerová, Zdenka and Skládal, Petr and Turánek, Jaroslav},
   article_location = {Oxford},
   article_number = {9-10},
   keywords = {Piezoelectric biosensor; Reisstance; Resonant frequency; Laminin; Fibronectin; Vitronectin},
   language = {eng},
   issn = {0956-5663},
   journal = {Biosensors & Bioelectronics},
   title = {Adhesion of eukaryotic cell lines on the gold surface modified with extracellular matrix proteins monitored by the piezoelectric sensor},
   url = {http://www.sciencedirect.com/dx.doi.org/10.1016/j.bios.2006.08.015},
   volume = {22},
   year = {2007}
}

TY  - JOUR
ID  - 701750
AU  - Fohlerová, Zdenka - Skládal, Petr - Turánek, Jaroslav
PY  - 2007
TI  - Adhesion of eukaryotic cell lines on the gold surface modified with extracellular matrix proteins monitored by the piezoelectric sensor
JF  - Biosensors & Bioelectronics
VL  - 22
IS  - 9-10
SP  - 1896-1901
EP  - 1896-1901
PB  - Elsevier Advanced Technology
SN  - 09565663
KW  - Piezoelectric biosensor
KW  - Reisstance
KW  - Resonant frequency
KW  - Laminin
KW  - Fibronectin
KW  - Vitronectin
UR  - http://www.sciencedirect.com/dx.doi.org/10.1016/j.bios.2006.08.015
N2  - The piezoelectric sensor (quartz crystal microbalance, QCM) was used to monitor cell adhesion in real time. Two cell lines, rat epithelial cells (WB F344) and lung melanoma cells (B16F10) were used. The cells were adhered and grown on the gold surface of the sensor pre-coated with adsorbed layer of extracellular matrix proteins as vitronectin and laminin. The process of cell attachment and spreading on the gold surface was continuously monitored and displayed by changes of the resonant frequency and resistance values of the piezoelectric resonators. The initial phase of cell attachment and spreading induced a decrease of frequency and increase of resistance relating viscoelastic properties of the cell monolayer on the sensing surface. The steady-state of both shifts was achieved after a few hours. The presence and state of cells on the surface was confirmed by fluorescent microscopy. The obtained results demonstrate that the piezoelectric sensor is suitable for studies of the cell adhesion processes. Thus obtained cell-based biosensor has potential for identification and screening of biologically active drugs and other biomolecules affecting cellular shape and attachment.
ER  -

FOHLEROVÁ, Zdenka, Petr SKLÁDAL and Jaroslav TURÁNEK. Adhesion of eukaryotic cell lines on the gold surface modified with extracellular matrix proteins monitored by the piezoelectric sensor. \textit{Biosensors \&{} Bioelectronics}. Oxford: Elsevier Advanced Technology, 2007, vol.~22, 9-10, p.~1896-1901. ISSN~0956-5663.

Detailed Information on Publication Record