| OČADLÍKOVÁ, Darina, Lenka ZAHRADOVÁ, Lucie KOVÁŘOVÁ, Miroslav PENKA, Jan ŽALOUDÍK, Roman HÁJEK and Jaroslav MICHÁLEK. Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy. Medical Oncology. Totowa, NJ, USA: Humana Press, 2006, 23/2006, No 3, p. 377-384. ISSN 1559-131X. |
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@article{702247,
author = {Očadlíková, Darina and Zahradová, Lenka and Kovářová, Lucie and Penka, Miroslav and Žaloudík, Jan and Hájek, Roman and Michálek, Jaroslav},
article_location = {Totowa, NJ, USA},
article_number = {3},
keywords = {Multiple myeloma; immunotherapy; interferon-gamma; T cell;CFSE},
language = {eng},
issn = {1559-131X},
journal = {Medical Oncology},
title = {Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy},
volume = {23/2006},
year = {2006}
}
TY - JOUR
ID - 702247
AU - Očadlíková, Darina - Zahradová, Lenka - Kovářová, Lucie - Penka, Miroslav - Žaloudík, Jan - Hájek, Roman - Michálek, Jaroslav
PY - 2006
TI - Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy
JF - Medical Oncology
VL - 23/2006
IS - 3
SP - 377-384
EP - 377-384
PB - Humana Press
SN - 1559131X
KW - Multiple myeloma
KW - immunotherapy
KW - interferon-gamma
KW - T cell;CFSE
N2 - Adoptive immunotherapy is a promising approach in the treatment of multiple myeloma. We have tested the identification, separation, and expansion of allogeneic myeloma-specific T cells in vitro. Irradiated myeloma cell line ARH 77 has been used to stimulate allogeneic CD4+ and CD8+ T lymphocytes. Activated myeloma-specific T cells that produced interferon-gamma were isolated using immunomagnetic beads and further expanded in vitro to numbers of up to 400 e06 T cells. Specificity of the T lymphocytes was tested using a 5-(6-)carboxyfluoresceine diacetate succinimidyl ester (CFSE)–based cytotoxicity test. This study demonstrates the feasibility of identification and isolation of tumor-specific T cells from allogeneic donors that can be expanded in vitro to numbers useful for clinical applications.
ER -
OČADLÍKOVÁ, Darina, Lenka ZAHRADOVÁ, Lucie KOVÁŘOVÁ, Miroslav PENKA, Jan ŽALOUDÍK, Roman HÁJEK and Jaroslav MICHÁLEK. Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy. \textit{Medical Oncology}. Totowa, NJ, USA: Humana Press, 2006, 23/2006, No~3, p.~377-384. ISSN~1559-131X.
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