OČADLÍKOVÁ, Darina, Lenka ZAHRADOVÁ, Lucie KOVÁŘOVÁ, Miroslav PENKA, Jan ŽALOUDÍK, Roman HÁJEK and Jaroslav MICHÁLEK. Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy. Medical Oncology. Totowa, NJ, USA: Humana Press, 2006, 23/2006, No 3, p. 377-384. ISSN 1559-131X. |
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@article{702247, author = {Očadlíková, Darina and Zahradová, Lenka and Kovářová, Lucie and Penka, Miroslav and Žaloudík, Jan and Hájek, Roman and Michálek, Jaroslav}, article_location = {Totowa, NJ, USA}, article_number = {3}, keywords = {Multiple myeloma; immunotherapy; interferon-gamma; T cell;CFSE}, language = {eng}, issn = {1559-131X}, journal = {Medical Oncology}, title = {Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy}, volume = {23/2006}, year = {2006} }
TY - JOUR ID - 702247 AU - Očadlíková, Darina - Zahradová, Lenka - Kovářová, Lucie - Penka, Miroslav - Žaloudík, Jan - Hájek, Roman - Michálek, Jaroslav PY - 2006 TI - Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy JF - Medical Oncology VL - 23/2006 IS - 3 SP - 377-384 EP - 377-384 PB - Humana Press SN - 1559131X KW - Multiple myeloma KW - immunotherapy KW - interferon-gamma KW - T cell;CFSE N2 - Adoptive immunotherapy is a promising approach in the treatment of multiple myeloma. We have tested the identification, separation, and expansion of allogeneic myeloma-specific T cells in vitro. Irradiated myeloma cell line ARH 77 has been used to stimulate allogeneic CD4+ and CD8+ T lymphocytes. Activated myeloma-specific T cells that produced interferon-gamma were isolated using immunomagnetic beads and further expanded in vitro to numbers of up to 400 e06 T cells. Specificity of the T lymphocytes was tested using a 5-(6-)carboxyfluoresceine diacetate succinimidyl ester (CFSE)–based cytotoxicity test. This study demonstrates the feasibility of identification and isolation of tumor-specific T cells from allogeneic donors that can be expanded in vitro to numbers useful for clinical applications. ER -
OČADLÍKOVÁ, Darina, Lenka ZAHRADOVÁ, Lucie KOVÁŘOVÁ, Miroslav PENKA, Jan ŽALOUDÍK, Roman HÁJEK and Jaroslav MICHÁLEK. Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy. \textit{Medical Oncology}. Totowa, NJ, USA: Humana Press, 2006, 23/2006, No~3, p.~377-384. ISSN~1559-131X.
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