Isolation and Expansion of Allogeneic Myeloma-Specific
Interferon-Gamma Producing T Cells for Adoptive Immunotherapy

J 2006

Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy

OČADLÍKOVÁ, Darina, Lenka ZAHRADOVÁ, Lucie KOVÁŘOVÁ, Miroslav PENKA, Jan ŽALOUDÍK et. al.

Basic information

Original name

Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy

Name in Czech

Izolace a expanze alogenních myelom-specifických a interferon gama-pozitivních T lymfocytů pro adoptivní imunoterapii

Authors

OČADLÍKOVÁ, Darina (203 Czech Republic, guarantor), Lenka ZAHRADOVÁ (203 Czech Republic), Lucie KOVÁŘOVÁ (203 Czech Republic), Miroslav PENKA (203 Czech Republic), Jan ŽALOUDÍK (203 Czech Republic), Roman HÁJEK (203 Czech Republic) and Jaroslav MICHÁLEK (203 Czech Republic)

Edition

Medical Oncology, Totowa, NJ, USA, Humana Press, 2006, 1559-131X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.256

RIV identification code

RIV/00216224:14110/06:00018909

Organization unit

Faculty of Medicine

UT WoS

000242136000009

Keywords in English

Multiple myeloma; immunotherapy; interferon-gamma; T cell;CFSE

Abstract

ORIG CZ

V originále

Adoptive immunotherapy is a promising approach in the treatment of multiple myeloma. We have tested the identification, separation, and expansion of allogeneic myeloma-specific T cells in vitro. Irradiated myeloma cell line ARH 77 has been used to stimulate allogeneic CD4+ and CD8+ T lymphocytes. Activated myeloma-specific T cells that produced interferon-gamma were isolated using immunomagnetic beads and further expanded in vitro to numbers of up to 400 e06 T cells. Specificity of the T lymphocytes was tested using a 5-(6-)carboxyfluoresceine diacetate succinimidyl ester (CFSE)–based cytotoxicity test. This study demonstrates the feasibility of identification and isolation of tumor-specific T cells from allogeneic donors that can be expanded in vitro to numbers useful for clinical applications.

In Czech

Izolace a expanze alogenních myelom-specifických interferon-gama produkujících T lymfocytů pro buněčnou imunoterapii u mnohočetného myelomu.

Links

NC7475, research and development project

Name: Hodnocení buněčné imunity a molekulární analýza myelom-specifických T lymfocytů u pacientů s mnohočetným myelomem

Citovat

OČADLÍKOVÁ, Darina, Lenka ZAHRADOVÁ, Lucie KOVÁŘOVÁ, Miroslav PENKA, Jan ŽALOUDÍK, Roman HÁJEK and Jaroslav MICHÁLEK. Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy. Medical Oncology. Totowa, NJ, USA: Humana Press, 2006, 23/2006, No 3, p. 377-384. ISSN 1559-131X.

@article{702247,
   author = {Očadlíková, Darina and Zahradová, Lenka and Kovářová, Lucie and Penka, Miroslav and Žaloudík, Jan and Hájek, Roman and Michálek, Jaroslav},
   article_location = {Totowa, NJ, USA},
   article_number = {3},
   keywords = {Multiple myeloma; immunotherapy; interferon-gamma; T cell;CFSE},
   language = {eng},
   issn = {1559-131X},
   journal = {Medical Oncology},
   title = {Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy},
   volume = {23/2006},
   year = {2006}
}

TY  - JOUR
ID  - 702247
AU  - Očadlíková, Darina - Zahradová, Lenka - Kovářová, Lucie - Penka, Miroslav - Žaloudík, Jan - Hájek, Roman - Michálek, Jaroslav
PY  - 2006
TI  - Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy
JF  - Medical Oncology
VL  - 23/2006
IS  - 3
SP  - 377-384
EP  - 377-384
PB  - Humana Press
SN  - 1559131X
KW  - Multiple myeloma
KW  - immunotherapy
KW  - interferon-gamma
KW  - T cell;CFSE
N2  - Adoptive immunotherapy is a promising approach in the treatment of multiple myeloma. We have tested the identification, separation, and expansion of allogeneic myeloma-specific T cells in vitro. Irradiated myeloma cell line ARH 77 has been used to stimulate allogeneic CD4+ and CD8+ T lymphocytes. Activated myeloma-specific T cells that produced interferon-gamma were isolated using immunomagnetic beads and further expanded in vitro to numbers of up to 400 e06 T cells. Specificity of the T lymphocytes was tested using a 5-(6-)carboxyfluoresceine diacetate succinimidyl ester (CFSE)–based cytotoxicity test. This study demonstrates the feasibility of identification and isolation of tumor-specific T cells from allogeneic donors that can be expanded in vitro to numbers useful for clinical applications.
ER  -

OČADLÍKOVÁ, Darina, Lenka ZAHRADOVÁ, Lucie KOVÁŘOVÁ, Miroslav PENKA, Jan ŽALOUDÍK, Roman HÁJEK and Jaroslav MICHÁLEK. Isolation and Expansion of Allogeneic Myeloma-Specific Interferon-Gamma Producing T Cells for Adoptive Immunotherapy. \textit{Medical Oncology}. Totowa, NJ, USA: Humana Press, 2006, 23/2006, No~3, p.~377-384. ISSN~1559-131X.

Detailed Information on Publication Record