Microtubular and actin cytoskeletons and ultrastructural
characteristics of the potentially pathogenic basidiomycetous
yeast Malassezia pachydermatis

J 2007

Microtubular and actin cytoskeletons and ultrastructural characteristics of the potentially pathogenic basidiomycetous yeast Malassezia pachydermatis

DAVID, Marek, Miroslav GABRIEL and Marie KOPECKÁ

Basic information

Original name

Microtubular and actin cytoskeletons and ultrastructural characteristics of the potentially pathogenic basidiomycetous yeast Malassezia pachydermatis

Authors

DAVID, Marek (203 Czech Republic, belonging to the institution), Miroslav GABRIEL (203 Czech Republic, belonging to the institution) and Marie KOPECKÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Cell Biology International, England, Elsevier, 2007, 1065-6995

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30000 3. Medical and Health Sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL publikace URL

Impact factor

Impact factor: 1.547

RIV identification code

RIV/00216224:14110/07:00020053

Organization unit

Faculty of Medicine

UT WoS

000244297300003

Keywords in English

actin; basidiomycetous yeasts; cytoskeleton; Malassezia pachydermatis; microtubules; ultrastructure

Abstract

V originále

Microtubular and actin cytoskeletons were investigated by fluorescence and electron microscopy. Microtubules were detected by indirect immunofluorescence. Cytoplasmic microtubules were detected in interphase and a spindle with astral microtubules was seen in M-phase. The disintegration of cytoplasmic microtubules and migration of the nucleus to the bud before mitosis were characteristic features of the basidiomycetous yeast Malassezia pachydermatis. The visualisation of F-actin structures (patches, cables and cytokinetic rings) by fluorescence microscopy using both monoclonal anti-actin antibody and rhodamine-phalloidin failed, but actin was detected by electron microscopy with immunogold labelling. Clusters of gold particles indicating actin structures were detected at the plasma membrane of cells with unique cortical ultrastructural features characteristic of the genus Malassezia. A possible association of these with the actin cytoskeleton is suggested.

Links

GA310/03/1195, research and development project

Name: Cytoskelet jako terčová struktura pro studium účinku antifungálních látek u patogenních kvasinek

Investor: Czech Science Foundation

GA310/06/0605, research and development project

Name: Lidské patogenní houby : cytoskelet jako potenciální terč inhibice morfogeneze.

Investor: Czech Science Foundation, Human pathogenic fungi: the cytoskeleton as a potential target of inhibition of

Citovat

DAVID, Marek, Miroslav GABRIEL and Marie KOPECKÁ. Microtubular and actin cytoskeletons and ultrastructural characteristics of the potentially pathogenic basidiomycetous yeast Malassezia pachydermatis. Cell Biology International. England: Elsevier, 2007, vol. 31, No 1, p. 16-23. ISSN 1065-6995.

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   author = {David, Marek and Gabriel, Miroslav and Kopecká, Marie},
   article_location = {England},
   article_number = {1},
   keywords = {actin; basidiomycetous yeasts; cytoskeleton; Malassezia pachydermatis; microtubules; ultrastructure},
   language = {eng},
   issn = {1065-6995},
   journal = {Cell Biology International},
   title = {Microtubular and actin cytoskeletons and ultrastructural characteristics of the potentially pathogenic basidiomycetous yeast Malassezia pachydermatis},
   url = {http://apps.isiknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=1&SID=V1bJDKhI2DbNlPJIE65&page=1&doc=5},
   volume = {31},
   year = {2007}
}

TY  - JOUR
ID  - 703506
AU  - David, Marek - Gabriel, Miroslav - Kopecká, Marie
PY  - 2007
TI  - Microtubular and actin cytoskeletons and ultrastructural characteristics of the potentially pathogenic basidiomycetous yeast Malassezia pachydermatis
JF  - Cell Biology International
VL  - 31
IS  - 1
SP  - 16-23
EP  - 16-23
PB  - Elsevier
SN  - 10656995
KW  - actin
KW  - basidiomycetous yeasts
KW  - cytoskeleton
KW  - Malassezia pachydermatis
KW  - microtubules
KW  - ultrastructure
UR  - http://apps.isiknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=1&SID=V1bJDKhI2DbNlPJIE65&page=1&doc=5
N2  - Microtubular and actin cytoskeletons were investigated by fluorescence and electron microscopy. Microtubules were detected by indirect immunofluorescence. Cytoplasmic microtubules were detected in interphase and a spindle with astral microtubules was seen in M-phase. The disintegration of cytoplasmic microtubules and migration of the nucleus to the bud before mitosis were characteristic features of the basidiomycetous yeast Malassezia pachydermatis. The visualisation of F-actin structures (patches, cables and cytokinetic rings) by fluorescence microscopy using both monoclonal anti-actin antibody and rhodamine-phalloidin failed, but actin was detected by electron microscopy with immunogold labelling. Clusters of gold particles indicating actin structures were detected at the plasma membrane of cells with unique cortical ultrastructural features characteristic of the genus Malassezia. A possible association of these with the actin cytoskeleton is suggested.
ER  -

DAVID, Marek, Miroslav GABRIEL and Marie KOPECKÁ. Microtubular and actin cytoskeletons and ultrastructural characteristics of the potentially pathogenic basidiomycetous yeast Malassezia pachydermatis. \textit{Cell Biology International}. England: Elsevier, 2007, vol.~31, No~1, p.~16-23. ISSN~1065-6995.

Detailed Information on Publication Record