Cytoskeletal structures, ultrastructural characteristics and
the capsule of the basidiomycetous yeast Cryptococcus laurentii

J 2007

Cytoskeletal structures, ultrastructural characteristics and the capsule of the basidiomycetous yeast Cryptococcus laurentii

DAVID, Marek, Miroslav GABRIEL and Marie KOPECKÁ

Basic information

Original name

Cytoskeletal structures, ultrastructural characteristics and the capsule of the basidiomycetous yeast Cryptococcus laurentii

Authors

DAVID, Marek (203 Czech Republic, belonging to the institution), Miroslav GABRIEL (203 Czech Republic, belonging to the institution) and Marie KOPECKÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology, Nizozemské království, Springer, 2007, 0003-6072

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30000 3. Medical and Health Sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

publikace URL

Impact factor

Impact factor: 1.547

RIV identification code

RIV/00216224:14110/07:00020054

Organization unit

Faculty of Medicine

UT WoS

000246102600004

Keywords in English

capsule; cell cycle; Cryptococcus laurentii; cytoskeleton; morphology; ultrastructure

Abstract

V originále

The cytoskeleton, capsule and cell ultrastructure were studied during the cell cycle of Cryptococcus laurentii. In an encapsulated strain, cytoplasmic microtubules and a mitotic spindle were detected. Mitosis was preceded by migration of the nucleus into the bud. F-actin failed to be visualised by rhodamine-phalloidin (RhPh) in encapsulated cells and therefore an acapsular strain was used. The following actin structures were found: actin dots, actin cables and cytokinetic ring. Ultrastructural studies showed the presence of a nucleus in the bud before mitosis. A collar-shaped structure was seen at the base of bud emergence. A lamellar cell wall and a rough outer surface of the cells were detected. Cytoskeletal structures found in Cryptococcus laurentii are similar to those in Cryptococcus neoformans, which is a serious human pathogen.

Links

GA310/03/1195, research and development project

Name: Cytoskelet jako terčová struktura pro studium účinku antifungálních látek u patogenních kvasinek

Investor: Czech Science Foundation

GA310/06/0605, research and development project

Name: Lidské patogenní houby : cytoskelet jako potenciální terč inhibice morfogeneze.

Investor: Czech Science Foundation, Human pathogenic fungi: the cytoskeleton as a potential target of inhibition of

Citovat

DAVID, Marek, Miroslav GABRIEL and Marie KOPECKÁ. Cytoskeletal structures, ultrastructural characteristics and the capsule of the basidiomycetous yeast Cryptococcus laurentii. Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology. Nizozemské království: Springer, 2007, vol. 92, No 1, p. 29-36. ISSN 0003-6072.

@article{703507,
   author = {David, Marek and Gabriel, Miroslav and Kopecká, Marie},
   article_location = {Nizozemské království},
   article_number = {1},
   keywords = {capsule; cell cycle; Cryptococcus laurentii; cytoskeleton; morphology; ultrastructure},
   language = {eng},
   issn = {0003-6072},
   journal = {Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology},
   title = {Cytoskeletal structures, ultrastructural characteristics and the capsule of the basidiomycetous yeast Cryptococcus laurentii},
   url = {http://www.springerlink.com/content/k15h23qpm2891309/},
   volume = {92},
   year = {2007}
}

TY  - JOUR
ID  - 703507
AU  - David, Marek - Gabriel, Miroslav - Kopecká, Marie
PY  - 2007
TI  - Cytoskeletal structures, ultrastructural characteristics and the capsule of the basidiomycetous yeast Cryptococcus laurentii
JF  - Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology
VL  - 92
IS  - 1
SP  - 29-36
EP  - 29-36
PB  - Springer
SN  - 00036072
KW  - capsule
KW  - cell cycle
KW  - Cryptococcus laurentii
KW  - cytoskeleton
KW  - morphology
KW  - ultrastructure
UR  - http://www.springerlink.com/content/k15h23qpm2891309/
N2  - The cytoskeleton, capsule and cell ultrastructure were studied during the cell cycle of Cryptococcus laurentii. In an encapsulated strain, cytoplasmic microtubules and a mitotic spindle were detected. Mitosis was preceded by migration of the nucleus into the bud. F-actin failed to be visualised by rhodamine-phalloidin (RhPh) in encapsulated cells and therefore an acapsular strain was used. The following actin structures were found: actin dots, actin cables and cytokinetic ring. Ultrastructural studies showed the presence of a nucleus in the bud before mitosis. A collar-shaped structure was seen at the base of bud emergence. A lamellar cell wall and a rough outer surface of the cells were detected. Cytoskeletal structures found in Cryptococcus laurentii are similar to those in Cryptococcus neoformans, which is a serious human pathogen.
ER  -

DAVID, Marek, Miroslav GABRIEL and Marie KOPECKÁ. Cytoskeletal structures, ultrastructural characteristics and the capsule of the basidiomycetous yeast Cryptococcus laurentii. \textit{Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology}. Nizozemské království: Springer, 2007, vol.~92, No~1, p.~29-36. ISSN~0003-6072.

Detailed Information on Publication Record