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@article{704159,
author = {Šmajs, David and Matějková, Petra and Weinstock, G.M.},
article_location = {United Kingdom},
article_number = {1},
keywords = {pore-forming colicin; colicin Y; colicin immunity; site-directed mutagenesis},
language = {eng},
issn = {0378-1097},
journal = {FEMS Microbiology Letters},
title = {Recognition of pore-forming colicin Y by its cognate immunity protein.},
volume = {258},
year = {2006}
}
TY - JOUR
ID - 704159
AU - Šmajs, David - Matějková, Petra - Weinstock, G.M.
PY - 2006
TI - Recognition of pore-forming colicin Y by its cognate immunity protein.
JF - FEMS Microbiology Letters
VL - 258
IS - 1
SP - 108-113
EP - 108-113
PB - Blackwell Publishing Ltd.
SN - 03781097
KW - pore-forming colicin
KW - colicin Y
KW - colicin immunity
KW - site-directed mutagenesis
N2 - Construction of hybrid immunity genes between colicin U (cui) and Y (cyi) immunity genes and site-directed mutagenesis of cyi were used to identify amino-acid residues of the colicin Y immunity protein (Cyi) involved in recognition of colicin Y. These amino-acid residues were localized close to the cytoplasmic site of the Cyi transmembrane helices T3 (S104, S107, F110, A112) and T4 (A159). Mutations in cui, which converted Cui sequence to Cyi sequence in positions 104, 107, 110, 112 and 159, resulted in an immunity gene that also conferred (besides immunity to colicin U) a high degree of immunity to colicin Y.
ER -
ŠMAJS, David, Petra MATĚJKOVÁ and G.M. WEINSTOCK. Recognition of pore-forming colicin Y by its cognate immunity protein. \textit{FEMS Microbiology Letters}. United Kingdom: Blackwell Publishing Ltd., 2006, vol.~258, No~1, p.~108-113. ISSN~0378-1097.
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