Occurence of strains producing specific anti-bacterial inhibitory agents in five genera of Enterobacteriaceae.

ŠMARDA, Jan, David ŠMAJS, Marie LHOTOVÁ and Daniela DĚDIČOVÁ. Occurence of strains producing specific anti-bacterial inhibitory agents in five genera of Enterobacteriaceae. Current Microbiology. New York: Springer Verlag, 2007, vol. 2007, No 54, p. 113-118, 7 pp. ISSN 0343-8651.

Basic information

• Original name: Occurence of strains producing specific anti-bacterial inhibitory agents in five genera of Enterobacteriaceae.
• Name in Czech: Výskyt kmenů produkujících specifické anti-bakteriální látky v 5 rodech čeledi Enterobacteriaceae.
• Name (in English): Occurence of strains producing specific anti-bacterial inhibitory agents in five genera of Enterobacteriaceae.
• Authors: ŠMARDA, Jan (203 Czech Republic), David ŠMAJS (203 Czech Republic, guarantor), Marie LHOTOVÁ (203 Czech Republic) and Daniela DĚDIČOVÁ (203 Czech Republic).
• Edition: Current Microbiology, New York, Springer Verlag, 2007, 0343-8651.

Other information

• Original language: Czech
• Type of outcome: Article in a journal
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 1.167
• RIV identification code: RIV/00216224:14110/07:00020057
• Organization unit: Faculty of Medicine
• UT WoS: 000243462300007
• Keywords in English: Escherichia coli; human intestinal strains; colicinogeny; lysogeny; siderophore
• Tags: colicinogeny, Escherichia coli, human intestinal strains, Lysogeny, siderophore

Abstract

Striking differences were found between genera of the family Enterobacteriaceae, concerning the extent of the capability of their strains to produce specific inhibitory agents interfering with viability of other strains of the same (or of related) species. We tested 50-163 strains each of the potentially pathogenic genera: Escherichia, Citrobacter, Enterobacter, Kluyvera and Leclercia for their ability to produce bacteriophages, high molecular weight- and low molecular weight-bacteriocins and siderophores against the same sets of strains, using the cross-test method. The genus Escherichia differs substantially from all other Enterobacteriaceae, harbouring a notable proportion of lysogenic (36.6%) and colicinogenic (13.9%) strains. Citrobacter strains are in 18.2% lysogenic and just sporadic ones colicinogenic, nevertheless in 7.3% produce phage tail-like bacteriocins. On the other hand, Kluyvera strains were only in 1.8% lysogenic, no colicinogenic was found, but in 7.3% produced siderophores causing zones of growth inhibition in agar cultures of strains of the same genus. In Leclercia, 10.0% of strains were lysogenic, 2.0% produced HMW bacteriocins, no colicinogenic was found and 2.0% produced siderophores. Enterobacter has shown 23.1% of strains producing siderophores, while merely 7.7% were lysogenic, 1.9% colicinogenic and 3.8% formed phage tail-like bacteriocins. These HMW bacteriocins disposed of a rather wide spectrum of activity. The siderophore activity spectrum was rather wide in any genus, but the siderophores were usually not secreted by strains producing phages or colicins.

Abstract (in English)

Striking differences were found between genera of the family Enterobacteriaceae, concerning the extent of the capability of their strains to produce specific inhibitory agents interfering with viability of other strains of the same (or of related) species. We tested 50-163 strains each of the potentially pathogenic genera: Escherichia, Citrobacter, Enterobacter, Kluyvera and Leclercia for their ability to produce bacteriophages, high molecular weight- and low molecular weight-bacteriocins and siderophores against the same sets of strains, using the cross-test method. The genus Escherichia differs substantially from all other Enterobacteriaceae, harbouring a notable proportion of lysogenic (36.6%) and colicinogenic (13.9%) strains. Citrobacter strains are in 18.2% lysogenic and just sporadic ones colicinogenic, nevertheless in 7.3% produce phage tail-like bacteriocins. On the other hand, Kluyvera strains were only in 1.8% lysogenic, no colicinogenic was found, but in 7.3% produced siderophores causing zones of growth inhibition in agar cultures of strains of the same genus. In Leclercia, 10.0% of strains were lysogenic, 2.0% produced HMW bacteriocins, no colicinogenic was found and 2.0% produced siderophores. Enterobacter has shown 23.1% of strains producing siderophores, while merely 7.7% were lysogenic, 1.9% colicinogenic and 3.8% formed phage tail-like bacteriocins. These HMW bacteriocins disposed of a rather wide spectrum of activity. The siderophore activity spectrum was rather wide in any genus, but the siderophores were usually not secreted by strains producing phages or colicins.

Links

• GA310/03/1091, research and development project: Name: Mapování interakce kolicinů s citlivou a imunní bakteriální buňkou: receptorové studie a inaktivace kolicinu jeho imunitním proteinem

Investor: Czech Science Foundation

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations