J 2007

Klinický materiál pro průkaz syfilis metodou polymerázové řetězové reakce.

WOZNICOVÁ, Vladana, Miroslav VOTAVA and Magdalena FLASAROVÁ

Basic information

Original name

Klinický materiál pro průkaz syfilis metodou polymerázové řetězové reakce.

Name in Czech

Klinický materiál pro průkaz syfilis metodou polymerázové řetězové reakce.

Name (in English)

Clinical specimens for PCR detection of syphilis

Authors

WOZNICOVÁ, Vladana (203 Czech Republic, guarantor), Miroslav VOTAVA (203 Czech Republic) and Magdalena FLASAROVÁ (203 Czech Republic)

Edition

Epidemiologie, mikrobiologie, imunologie. Praha, Česká lékařská společnost J. E. Purkyně, 2007, 1210-7913

Other information

Language

Czech

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14110/07:00028185

Organization unit

Faculty of Medicine

Keywords in English

syphilis; PCR; clinical samples
Změněno: 31/3/2010 12:16, doc. MUDr. Vladana Woznicová, Ph.D.

Abstract

V originále

A review is presented of types of clinical specimens used for diagnosis of syphilis by polymerase chain reaction. PCR is a routine method for detection of T. pallidum in swabs of chancres and primary and secondary syphilis mucocutaneous lesions. Whole blood, cerebrospinal fluid, amniotic fluid, aspirate and biopsy specimens, and paraffin-embedded tissues can also be tested by PCR for T. pallidum. However, further research on PCR detection sensitivity at various stages of syphilis is needed before these specimens are used in clinical practice.

In English

A review is presented of types of clinical specimens used for diagnosis of syphilis by polymerase chain reaction. PCR is a routine method for detection of T. pallidum in swabs of chancres and primary and secondary syphilis mucocutaneous lesions. Whole blood, cerebrospinal fluid, amniotic fluid, aspirate and biopsy specimens, and paraffin-embedded tissues can also be tested by PCR for T. pallidum. However, further research on PCR detection sensitivity at various stages of syphilis is needed before these specimens are used in clinical practice.

Links

NR8967, research and development project
Name: Molekulární typizace kmenů a izolátů Treponema pallidum subsp. pallidum: základ pro epidemiologickou a klinickou identifikaci jednotlivých kmenů
Investor: Ministry of Health of the CR