Simultaneous HPLC determination of markers of metabolic activity of CYP1A2 and CYP2C6

JUŘICA, Jan, Josef TOMANDL, Lucia ZAHRADNÍKOVÁ, Ondřej ZENDULKA and Eva HADAŠOVÁ. Simultaneous HPLC determination of markers of metabolic activity of CYP1A2 and CYP2C6. In Book of abstracts, Central/Eastern European CINP Regional meeting. Tallin: University of Tartu, 2006, p. 37-38, 1 pp.

Basic information

Original name

Simultaneous HPLC determination of markers of metabolic activity of CYP1A2 and CYP2C6

Name in Czech

Současné stanovení metabolické aktivity CYP1A2 a CYP2C6 pomocí HPLC

Authors

JUŘICA, Jan, Josef TOMANDL, Lucia ZAHRADNÍKOVÁ, Ondřej ZENDULKA and Eva HADAŠOVÁ

Edition

Tallin, Book of abstracts, Central/Eastern European CINP Regional meeting, p. 37-38, 1 pp. 2006

Publisher

University of Tartu

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Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

30104 Pharmacology and pharmacy

Country of publisher

Estonia

Confidentiality degree

není předmětem státního či obchodního tajemství

Organization unit

Faculty of Medicine

Keywords in English

determination; cytochrome; CYP2C6; CYP1A2

Tags

CYP1A2, CYP2C6, cytochrome, determination

Tags

International impact

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Abstract

V originále

The presented method allows to determine concentration of both markers of metabolic activity of CYP1A and CYP2C (phenacetine and tolbutamide) and their metabolites paracetamol and 4-hydroxytolbutamide in rat liver perfusate, respectively. This method is suitable for modeling and description of possible pharmacological interactions on isoforms CYP1A2 and CYP2C6 in rat.

In Czech

Metoda umožňuje současně stanovit 2 markery metabolické aktivity CYP1A2 a CYP29 (fenacetin a tolbutamid), respektive současně i jejich metabolity (paracetamol, hydroxytolbutamid) v perfuzním mediu. Metoda je vhodná pro popis a modelování farmakokinetických interakcí na potkaních isoformách CYP1A2 a 2C9 cytochromu P450.

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Links

GA203/06/0047, research and development project	Name: Využití kapilární elektroforézy pro studium metabolismu léčiv Investor: Czech Science Foundation, Capillary electrophoresis as a tool for the drug-metabolism studies
LC06023, research and development project	Name: Integrované bioanalytické technologie pro mikroanalýzy a diagnostiku s využitím LIF a hmotnostní spektrometrie Investor: Ministry of Education, Youth and Sports of the CR
MSM0021622404, plan (intention)	Name: Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS Investor: Ministry of Education, Youth and Sports of the CR, The internal organisation and neurobiological mechanisms of functional CNS systems under normal and pathological conditions.