PEJCHALOVÁ, Kateřina, Alena ŽÁKOVSKÁ, Marie MEJZLÍKOVÁ, Jiří HALOUZKA and Miloš DENDIS. Isolation, Cultivation and Identification of Borrelia burgdorferi genospecies from Ixodes ricinus ticks from South Moravia, Czech Republic. Annals of Agricultural and Environmental Medicine. 2007, vol. 14, No 1, p. 75-79. ISSN 1232-1966. |
Other formats:
BibTeX
LaTeX
RIS
@article{704353, author = {Pejchalová, Kateřina and Žákovská, Alena and Mejzlíková, Marie and Halouzka, Jiří and Dendis, Miloš}, article_number = {1}, keywords = {Borrelia burgdorferi; Ixodes ricinus; DFM; PCR-RFLP}, language = {eng}, issn = {1232-1966}, journal = {Annals of Agricultural and Environmental Medicine}, title = {Isolation, Cultivation and Identification of Borrelia burgdorferi genospecies from Ixodes ricinus ticks from South Moravia, Czech Republic.}, volume = {14}, year = {2007} }
TY - JOUR ID - 704353 AU - Pejchalová, Kateřina - Žákovská, Alena - Mejzlíková, Marie - Halouzka, Jiří - Dendis, Miloš PY - 2007 TI - Isolation, Cultivation and Identification of Borrelia burgdorferi genospecies from Ixodes ricinus ticks from South Moravia, Czech Republic. JF - Annals of Agricultural and Environmental Medicine VL - 14 IS - 1 SP - 75-79 EP - 75-79 SN - 12321966 KW - Borrelia burgdorferi KW - Ixodes ricinus KW - DFM KW - PCR-RFLP N2 - The prevalence of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from South Moravia was investigated. A total of 305 ticks (21 larvae, 243 nymphs, 19 females and 22 males) were collected in suburban woods of Pisárky Park (city of Brno) from July to October 2002. All specimens were examined by dark-field microscopy (DFM) for the presence of spirochetes. The mean infection rate was 14.8%. 13.6% of males and 26.3% of females tested were positive for the presence of B. burgdorferi s.l.. Also, of the 243 nymphs, 14.4% were positive and out of 21 larvae 9.5% were positive. Borreliae were identified by polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) methods. From a total of 45 DFM positive samples 37 were positive for the presence of borrelian DNA. Isolation attempts resulted in 21 spirochaetal strains. Identification by PCR-RFLP detection revealed genospecies: Borrelia afzelii, B. garinii, B. burgdorferi sensu stricto and B. valaisiana. Four isolated strains showed a mixed population of these genospecies. ER -
PEJCHALOVÁ, Kateřina, Alena ŽÁKOVSKÁ, Marie MEJZLÍKOVÁ, Jiří HALOUZKA and Miloš DENDIS. Isolation, Cultivation and Identification of Borrelia burgdorferi genospecies from Ixodes ricinus ticks from South Moravia, Czech Republic. \textit{Annals of Agricultural and Environmental Medicine}. 2007, vol.~14, No~1, p.~75-79. ISSN~1232-1966.
|