Detailed Information on Publication Record
2007
Bioinformatic and image analyses of the cellular localization of the apoptotic proteins endonuclease G, AIF, and AMID during apoptosis in human cells
VAŘECHA, Miroslav, Jana AMRICHOVÁ, Michal ZIMMERMANN, Vladimír ULMAN, Emilie LUKÁŠOVÁ et. al.Basic information
Original name
Bioinformatic and image analyses of the cellular localization of the apoptotic proteins endonuclease G, AIF, and AMID during apoptosis in human cells
Name in Czech
Bioinformatická a obrazová analýza buněčné lokalizace apoptotických proteinů endonukleázy G, AIF a AMIDu během apoptózy v lidských buňkách
Authors
VAŘECHA, Miroslav (203 Czech Republic, guarantor, belonging to the institution), Jana AMRICHOVÁ (203 Czech Republic, belonging to the institution), Michal ZIMMERMANN (203 Czech Republic, belonging to the institution), Vladimír ULMAN (203 Czech Republic, belonging to the institution), Emilie LUKÁŠOVÁ (203 Czech Republic) and Michal KOZUBEK (203 Czech Republic, belonging to the institution)
Edition
Apoptosis, USA, Springer Science, 2007, 1360-8185
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
Genetics and molecular biology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 3.043
RIV identification code
RIV/00216224:14330/07:00020060
Organization unit
Faculty of Informatics
UT WoS
000247184000003
Keywords in English
apoptosis-inducing factor; endonuclease G; AIF-homologous mitochondrion-associated inducer of death; living cells; colocalization image analysis; analysis of amino acid sequence
Tags
Tags
International impact, Reviewed
Změněno: 20/6/2012 09:03, RNDr. Vladimír Ulman, Ph.D.
V originále
We studied the cellular localization of the apoptotic proteins endonuclease G, AIF, and AMID in silico using three prediction tools and in living cells using both single-cell colocalization image analysis and nuclear translocation analysis. We confirmed the mitochondrial localization of endonuclease G and AIF by prediction analysis and by single-cell colocalization image analysis. We found the AMID protein to be cytoplasmic, most probably incorporated into the cytoplasmic side of the membranes of various organelles. The highest concentration of AMID was observed associated with the Golgi. Colocalization of AMID with lysosomes was also indirectly confirmed by analysis of AMID-rich vesicle velocity using manual tracking analysis. Bioinformatic analysis also detected nuclear localization signals in endonuclease G and AIF, but not in AMID. A novel analysis of time-lapse fluorescence image data during staurosporine-induced apoptosis revealed nuclear translocation only for endonuclease G and AIF.
In Czech
Bioinformatická a obrazová analýza buněčné lokalizace apoptotických proteinů endonukleázy G, AIF a AMIDu během apoptózy v lidských buňkách
Links
| GA202/04/0907, research and development project |
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| GP204/03/D031, research and development project |
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| GP204/05/P090, research and development project |
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| LC535, research and development project |
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| MSM0021622419, plan (intention) |
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