| VAŘECHA, Miroslav, Jana AMRICHOVÁ, Michal ZIMMERMANN, Vladimír ULMAN, Emilie LUKÁŠOVÁ and Michal KOZUBEK. Bioinformatic and image analyses of the cellular localization of the apoptotic proteins endonuclease G, AIF, and AMID during apoptosis in human cells. Apoptosis. USA: Springer Science, 2007, vol. 12, No 7, p. 1155-1171. ISSN 1360-8185. |
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@article{704400,
author = {Vařecha, Miroslav and Amrichová, Jana and Zimmermann, Michal and Ulman, Vladimír and Lukášová, Emilie and Kozubek, Michal},
article_location = {USA},
article_number = {7},
keywords = {apoptosis-inducing factor; endonuclease G; AIF-homologous mitochondrion-associated inducer of death; living cells; colocalization image analysis; analysis of amino acid sequence},
language = {eng},
issn = {1360-8185},
journal = {Apoptosis},
title = {Bioinformatic and image analyses of the cellular localization of the apoptotic proteins endonuclease G, AIF, and AMID during apoptosis in human cells},
volume = {12},
year = {2007}
}
TY - JOUR
ID - 704400
AU - Vařecha, Miroslav - Amrichová, Jana - Zimmermann, Michal - Ulman, Vladimír - Lukášová, Emilie - Kozubek, Michal
PY - 2007
TI - Bioinformatic and image analyses of the cellular localization of the apoptotic proteins endonuclease G, AIF, and AMID during apoptosis in human cells
JF - Apoptosis
VL - 12
IS - 7
SP - 1155-1171
EP - 1155-1171
PB - Springer Science
SN - 13608185
KW - apoptosis-inducing factor
KW - endonuclease G
KW - AIF-homologous mitochondrion-associated inducer of death
KW - living cells
KW - colocalization image analysis
KW - analysis of amino acid sequence
N2 - We studied the cellular localization of the apoptotic proteins endonuclease G, AIF, and AMID in silico using three prediction tools and in living cells using both single-cell colocalization image analysis and nuclear translocation analysis. We confirmed the mitochondrial localization of endonuclease G and AIF by prediction analysis and by single-cell colocalization image analysis. We found the AMID protein to be cytoplasmic, most probably incorporated into the cytoplasmic side of the membranes of various organelles. The highest concentration of AMID was observed associated with the Golgi. Colocalization of AMID with lysosomes was also indirectly confirmed by analysis of AMID-rich vesicle velocity using manual tracking analysis. Bioinformatic analysis also detected nuclear localization signals in endonuclease G and AIF, but not in AMID. A novel analysis of time-lapse fluorescence image data during staurosporine-induced apoptosis revealed nuclear translocation only for endonuclease G and AIF.
ER -
VAŘECHA, Miroslav, Jana AMRICHOVÁ, Michal ZIMMERMANN, Vladimír ULMAN, Emilie LUKÁŠOVÁ and Michal KOZUBEK. Bioinformatic and image analyses of the cellular localization of the apoptotic proteins endonuclease G, AIF, and AMID during apoptosis in human cells. \textit{Apoptosis}. USA: Springer Science, 2007, vol.~12, No~7, p.~1155-1171. ISSN~1360-8185.
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