SLANINOVÁ, Iva, Zdenka SLUNSKÁ, Jiří ŠINKORA, Marcela VLKOVÁ and Eva TÁBORSKÁ. Screening of minor benzo(c)phenanthridine alkaloids for antiproliferative and apoptotic activities. Pharmaceutical Biology. NETHERLANDS: TAYLOR & FRANCIS INC, 2007, vol. 45, No 2, p. 131-139, 10 pp. ISSN 1388-0209.
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Basic information
Original name Screening of minor benzo(c)phenanthridine alkaloids for antiproliferative and apoptotic activities.
Name in Czech Skrining benzo(c)phenanthridinových alkaloidů na antiproliferativní a proapoptotické vlastnosti
Authors SLANINOVÁ, Iva (203 Czech Republic, guarantor), Zdenka SLUNSKÁ (203 Czech Republic), Jiří ŠINKORA (203 Czech Republic), Marcela VLKOVÁ (203 Czech Republic) and Eva TÁBORSKÁ (203 Czech Republic).
Edition Pharmaceutical Biology, NETHERLANDS, TAYLOR & FRANCIS INC, 2007, 1388-0209.
Other information
Original language English
Type of outcome Article in a journal
Field of Study Genetics and molecular biology
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
Impact factor Impact factor: 0.364
RIV identification code RIV/00216224:14110/07:00020083
Organization unit Faculty of Medicine
UT WoS 000245677200009
Keywords in English apoptosis; annexin V; benzo[c]phenanthridine alkaloids; cytotoxicity; chelerythrine; chelirubine; macarpine; MTT assay; sanguinarine; sanguirubine
Tags annexin V, apoptosis, Benzo[c]phenanthridine Alkaloids, Chelerythrine, Chelirubine, cytotoxicity, Macarpine, MTT assay, sanguinarine, Sanguirubine
Tags International impact, Reviewed
Changed by Changed by: prof. MUDr. Iva Slaninová, Ph.D., učo 2105. Changed: 22. 6. 2009 12:06.
Abstract
Quaternary benzo[c]phenanthridine alkaloids are a relatively small group of isoquinoline alkaloids with attractive biological activities. They are produced by a number of plant species of the Papaveraceae, Fumariaceae and Rutaceae families. Differential cytotoxicity of minor naturally occurring derivatives sanguirubine, chelirubine and macarpine and better known benzophenanthridines sanguinarine and chelerythrine in cancer and normal cells was assessed in vitro by MTT assay using a panel consisting of either transformed cell lines (HeLa; A431; HL60) or primary fibroblasts of human origin. IC50 values were determined 72 h after addition of the alkaloids. A wide range (0.01-1.44 mg/ml) of IC50 was observed and the highest toxicity was determined for macarpine. Human promyelocytic leukemia line HL60 has been documented the most sensitive to alkaloid treatment followed by human skin fibroblasts, while human cervix adenocarcinoma HeLa cells and epidermal carcinoma cells A431 appeared more resistant. The most sensitive cell line HL60 were selected for demonstrating the ability of the alkaloids to induce apoptosis by analysing morphological changes upon DAPI staining, ultrastructural changes and annexin V versus propidium iodide staining assay. The apoptosis was induced by sanguirubine, chelirubine, chelerythrine and macarpine at the concentration of 1 mg/ml. The results show that the alkaloids tested have strong antiproliferative effect in vitro due predominantly to apoptosis.
Abstract (in Czech)
Byly studovány antiproliferační a proapoptotické vlastnosti benzophenanthridinových alkaloidů.
Links
GA525/04/0017, research and development projectName: Rostlinné zdroje benzofenanthridinových alkaloidů a účinek těchto alkaloidů na normální a nádorové buňky
Investor: Czech Science Foundation, Plant sources of benzophenanthridine alkaloids and effects of these alkaloid on normal and cancer cells
GD301/03/H005, research and development projectName: Molekulární a buněčná biologie v biomedicíně
Investor: Czech Science Foundation, Molecular and cell biology in biomedicine
MSM0021622415, plan (intention)Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
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