Detailed Information on Publication Record
2007
Screening of minor benzo(c)phenanthridine alkaloids for antiproliferative and apoptotic activities.
SLANINOVÁ, Iva, Zdenka SLUNSKÁ, Jiří ŠINKORA, Marcela VLKOVÁ, Eva TÁBORSKÁ et. al.Basic information
Original name
Screening of minor benzo(c)phenanthridine alkaloids for antiproliferative and apoptotic activities.
Name in Czech
Skrining benzo(c)phenanthridinových alkaloidů na antiproliferativní a proapoptotické vlastnosti
Authors
SLANINOVÁ, Iva (203 Czech Republic, guarantor), Zdenka SLUNSKÁ (203 Czech Republic), Jiří ŠINKORA (203 Czech Republic), Marcela VLKOVÁ (203 Czech Republic) and Eva TÁBORSKÁ (203 Czech Republic)
Edition
Pharmaceutical Biology, NETHERLANDS, TAYLOR & FRANCIS INC, 2007, 1388-0209
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
Genetics and molecular biology
Country of publisher
Netherlands
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 0.364
RIV identification code
RIV/00216224:14110/07:00020083
Organization unit
Faculty of Medicine
UT WoS
000245677200009
Keywords in English
apoptosis; annexin V; benzo[c]phenanthridine alkaloids; cytotoxicity; chelerythrine; chelirubine; macarpine; MTT assay; sanguinarine; sanguirubine
Tags
Tags
International impact, Reviewed
Změněno: 22/6/2009 12:06, prof. MUDr. Iva Slaninová, Ph.D.
V originále
Quaternary benzo[c]phenanthridine alkaloids are a relatively small group of isoquinoline alkaloids with attractive biological activities. They are produced by a number of plant species of the Papaveraceae, Fumariaceae and Rutaceae families. Differential cytotoxicity of minor naturally occurring derivatives sanguirubine, chelirubine and macarpine and better known benzophenanthridines sanguinarine and chelerythrine in cancer and normal cells was assessed in vitro by MTT assay using a panel consisting of either transformed cell lines (HeLa; A431; HL60) or primary fibroblasts of human origin. IC50 values were determined 72 h after addition of the alkaloids. A wide range (0.01-1.44 mg/ml) of IC50 was observed and the highest toxicity was determined for macarpine. Human promyelocytic leukemia line HL60 has been documented the most sensitive to alkaloid treatment followed by human skin fibroblasts, while human cervix adenocarcinoma HeLa cells and epidermal carcinoma cells A431 appeared more resistant. The most sensitive cell line HL60 were selected for demonstrating the ability of the alkaloids to induce apoptosis by analysing morphological changes upon DAPI staining, ultrastructural changes and annexin V versus propidium iodide staining assay. The apoptosis was induced by sanguirubine, chelirubine, chelerythrine and macarpine at the concentration of 1 mg/ml. The results show that the alkaloids tested have strong antiproliferative effect in vitro due predominantly to apoptosis.
In Czech
Byly studovány antiproliferační a proapoptotické vlastnosti benzophenanthridinových alkaloidů.
Links
| GA525/04/0017, research and development project |
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| GD301/03/H005, research and development project |
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| MSM0021622415, plan (intention) |
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