ONDROUŠKOVÁ, Eva, Karolína POVOLNÁ, Petr VÁŇA, Petr BENEŠ, Hana KONEČNÁ, Zbyněk ZDRÁHAL and Jan ŠMARDA. A proteomic analysis of protein variations during differentiation of v-myb-transformed monoblasts. Leukemia Research. Elsevier, 2007, vol. 31, No 2, p. 221-229. ISSN 0145-2126. |
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@article{705140, author = {Ondroušková, Eva and Povolná, Karolína and Váňa, Petr and Beneš, Petr and Konečná, Hana and Zdráhal, Zbyněk and Šmarda, Jan}, article_number = {2}, keywords = {v-myb; monoblast; macrophage; differentiation; proteome; two-dimensional gel electrophoresis; trichostatin A; TPA; mass spectrometry}, language = {eng}, issn = {0145-2126}, journal = {Leukemia Research}, title = {A proteomic analysis of protein variations during differentiation of v-myb-transformed monoblasts}, volume = {31}, year = {2007} }
TY - JOUR ID - 705140 AU - Ondroušková, Eva - Povolná, Karolína - Váňa, Petr - Beneš, Petr - Konečná, Hana - Zdráhal, Zbyněk - Šmarda, Jan PY - 2007 TI - A proteomic analysis of protein variations during differentiation of v-myb-transformed monoblasts JF - Leukemia Research VL - 31 IS - 2 SP - 221-229 EP - 221-229 PB - Elsevier SN - 01452126 KW - v-myb KW - monoblast KW - macrophage KW - differentiation KW - proteome KW - two-dimensional gel electrophoresis KW - trichostatin A KW - TPA KW - mass spectrometry N2 - v-myb-oncogene of avian myeloblastosis virus transforms myelomonocytic cells in vitro and induces acute monoblastic leukemia in vivo. The transforming effect of the v-myb can be suppressed using phorbol ester (TPA) or histone deacetylase inhibitor trichostatin A (TSA), the inducers of cell differentiation that are in clinical trials. In this study, we used proteomics-based approach to identify proteins with variable expression in differentiated BM2 cells. Proteome variations induced by TPA and TSA were compared to examine the mechanism of differentiation-promoting effects of these drugs. We found that expression of several proteins participating in cell cytoskeleton rearrangement, heat shock response, proteosynthesis and cell signaling was altered in TPA- or TSA-treated cells. We present here the first comparative proteome analysis of v-myb-transformed monoblasts BM2 focused on identification of proteins involved in their terminal differentiation. ER -
ONDROUŠKOVÁ, Eva, Karolína POVOLNÁ, Petr VÁŇA, Petr BENEŠ, Hana KONEČNÁ, Zbyněk ZDRÁHAL and Jan ŠMARDA. A proteomic analysis of protein variations during differentiation of v-myb-transformed monoblasts. \textit{Leukemia Research}. Elsevier, 2007, vol.~31, No~2, p.~221-229. ISSN~0145-2126.
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