Fish mapping of ribosomal DNA and telomeric repeats in model species of Platyhelmithes and Acanthocephala

BOMBAROVÁ, Marta, Magda VÍTKOVÁ, František MAREC, Martina PEČÍNKOVÁ, Marta ŠPAKULOVÁ and Ivica HROMADOVÁ. Fish mapping of ribosomal DNA and telomeric repeats in model species of Platyhelmithes and Acanthocephala. In 11th International Congress of Parasitology, CD-ROM. Glasgow, Scotland: ICOPA XI Congress Secretariat, 2006, p. CD-ROM, 1 pp.

Basic information

• Original name: Fish mapping of ribosomal DNA and telomeric repeats in model species of Platyhelmithes and Acanthocephala
• Name in Czech: Cytogenetické mapování pomocí FISH ribozomální DNA a repetetivních telomerických sekvencí modelových parazitických druhů kmene Plathelminthes a Acanthocephala
• Authors: BOMBAROVÁ, Marta (703 Slovakia), Magda VÍTKOVÁ (203 Czech Republic), František MAREC (203 Czech Republic), Martina PEČÍNKOVÁ (203 Czech Republic, guarantor), Marta ŠPAKULOVÁ (703 Slovakia) and Ivica HROMADOVÁ (703 Slovakia).
• Edition: Glasgow, Scotland, 11th International Congress of Parasitology, CD-ROM, p. CD-ROM, 1 pp. 2006.
• Publisher: ICOPA XI Congress Secretariat

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: Genetics and molecular biology
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• Organization unit: Faculty of Science
• Keywords in English: FISH;Platyhelmithes;Acanthocephala
• Tags: Acanthocephala, FISH, Platyhelmithes

Abstract

Molecular cytogenetics is little exploited in metazoan parasites despite considerable relevance of the karyotype structure and chromosomal location of specific DNA sequences to phylogenetic and systematic evaluations. We applied fluorescence in situ hybridization (FISH) to determine the type of telomeric DNA and localize rDNA genes in chromosomes of several acantocephalan, monogenean and cestode species. In Paradiplozoon homoion-complex (Monogenea, 2n = 12), the chromosome pair no. 5 carried a nucleolar organizer region (NOR) composed of a cluster of rDNA genes as confirmed by both the classical banding approaches and FISH with 18S rDNA probe. In two acanthocephalan sibling species Pomphorhynchus laevis and P. tereticollis (Acanthocephala, both 2n = 6 + XX in female and X0 in male), two NORs were found, located each in different sites of the first two autosomal pairs. Furthermore, we hybridized the "vertebrate" telomeric probe, (TTAGGG)n, with chromosomes of above-mentioned helminths and cestodes Caryophyllaeus laticeps(2n = 20), Caryophyllaeides fennica (2n = 20), and Nippotaenia mogurndae (2n = 28). The probe hybridized with chromosome ends in all Platyhelminthes (Monogenea, Cestoda) but not in Acanthocephala. No hybridization signals in P. laevis and P. tereticollis were also obtained with the "arthropod" (TTAGG)n or "nematode"(TTAGGC)n telomeric probes. Our results suggest that whereas the telomeres in Platyhelminthes are composed of the TTAGGG repeates, this ancestral motif of telomeres in Bilateria was lost in Acanthocephala and replaced with unknown telomeric sequences.

Abstract (in Czech)

Byla aplikována fluorescence in situ hybridizace (FISH) pro stanovení typu telomerické DNA a lokalizace rDNA genů chromozomů několika druhů z taxonů Acanthocephala, Monogenea a Cestoda.

Links

• MSM0021622416, plan (intention): Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time