RITTICH, Bohuslav, Jana KŘÍŽOVÁ, Alena ŠPANOVÁ, Petra NĚMCOVÁ and Daniel HORÁK. Influence of magnetic particles on the reproducibility of RAPD and rep-PCR fingerprinting of lactic acid bacteria. In Ninth International Symposium on hyphenated techniques in chromatography and hyphenated chromatographic analyzers, HTC-9. York, Velká Británie: Royal Society of Chemistry, 2006, p. P167, 1 pp. |
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@inproceedings{706303, author = {Rittich, Bohuslav and Křížová, Jana and Španová, Alena and Němcová, Petra and Horák, Daniel}, address = {York, Velká Británie}, booktitle = {Ninth International Symposium on hyphenated techniques in chromatography and hyphenated chromatographic analyzers, HTC-9}, keywords = {Magnetic particles; HEMA-co-GMA; Bifidobacterium; RAPD; rep-PCR; reproducibility}, language = {eng}, location = {York, Velká Británie}, pages = {P167}, publisher = {Royal Society of Chemistry}, title = {Influence of magnetic particles on the reproducibility of RAPD and rep-PCR fingerprinting of lactic acid bacteria}, year = {2006} }
TY - JOUR ID - 706303 AU - Rittich, Bohuslav - Křížová, Jana - Španová, Alena - Němcová, Petra - Horák, Daniel PY - 2006 TI - Influence of magnetic particles on the reproducibility of RAPD and rep-PCR fingerprinting of lactic acid bacteria PB - Royal Society of Chemistry CY - York, Velká Británie KW - Magnetic particles KW - HEMA-co-GMA KW - Bifidobacterium KW - RAPD KW - rep-PCR KW - reproducibility N2 - DNA was isolated from Bifidobacterium cells using phenol extraction metod, salting out using 6 M NaCl, and adsorption on magnetic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (HEMA-co-GMA) particles containing carboxyl groups. It was shown that DNA eluted from magnetic particles into TE buffer can be succesfully used in molecular fingerprinting procedures. The reproducibility of tested methods were comparable with phenol extraction method. ER -
RITTICH, Bohuslav, Jana KŘÍŽOVÁ, Alena ŠPANOVÁ, Petra NĚMCOVÁ and Daniel HORÁK. Influence of magnetic particles on the reproducibility of RAPD and rep-PCR fingerprinting of lactic acid bacteria. In \textit{Ninth International Symposium on hyphenated techniques in chromatography and hyphenated chromatographic analyzers, HTC-9}. York, Velká Británie: Royal Society of Chemistry, 2006, p.~P167, 1 pp.
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