Different pattern of immunohistochemical localization of cannabinoid receptor CB2 in the rat dorsal root ganglia by two antibodies

SVÍŽENSKÁ, Ivana, Ilona KLUSÁKOVÁ and Petr DUBOVÝ. Different pattern of immunohistochemical localization of cannabinoid receptor CB2 in the rat dorsal root ganglia by two antibodies. In Morphology 2006. Praha: UK Praha, 2006, p. 158-158.

Basic information

• Original name: Different pattern of immunohistochemical localization of cannabinoid receptor CB2 in the rat dorsal root ganglia by two antibodies
• Name in Czech: Rozdílná reakce kanabinoidního receptoru CB2 ve spinálním gangliu potkana se dvěma protilátkami
• Authors: SVÍŽENSKÁ, Ivana, Ilona KLUSÁKOVÁ and Petr DUBOVÝ.
• Edition: Praha, Morphology 2006, p. 158-158, 1 pp. 2006.
• Publisher: UK Praha

Other information

• Original language: English
• Type of outcome: Proceedings paper
• Field of Study: 30000 3. Medical and Health Sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• Organization unit: Faculty of Medicine
• Keywords in English: cannabinoid receptor - neuropathic pain
• Tags: International impact, Reviewed

Abstract

CB2 receptor activation inhibits pain responses without adverse, most often psychotropic effects that are produced by CB1 agonists. We demonstrated different immunostaining for CB2 receptor in the DRG of adult rats by two antibodies raised against different epitopes of CB2. Rats were operated for unilateral sciatic nerve ligation and allowed to survive for 1 week. Longitudinal cryostat sections through ipsi-and contralateral L4 and L5 DRG were incubated with different primary antibodies. Immunofluorescence for CB2 receptors was observed predominantly in the nuclei of the DRG neurons when a rabbit polyclonal antibody raised against CB2 of human origin was used as the primary antibody. Using a goat polyclonal antibody raised against CB2 of mouse origin displayed immunostaining in the cytoplasm of the DRG neurons whereas nuclei were not stained. Total immunoreactivity of both antibodies was increased in the neurons of both ipsi- and contralateral DRG comparing with intact ones.

Abstract (in Czech)

Byly získány rozdílné imunohistochemické reakce ve spinálních gangliích potkana při použití dvou protilátek proti kanabinoidnímu receptoru CB2.

Links

• MSM0021622404, plan (intention): Name: Vnitřní organizace a neurobiologické mechanismy funkčních systémů CNS

Investor: Ministry of Education, Youth and Sports of the CR, The internal organisation and neurobiological mechanisms of functional CNS systems under normal and pathological conditions.