Detailed Information on Publication Record
2006
Taxonomic analysis and reclassification of Bifidobacterium adolescentis CCM 3761
ŠPANOVÁ, Alena, Jana KŘÍŽOVÁ, Radka BURDYCHOVÁ, Vladimír DRÁB, Bohuslav RITTICH et. al.Basic information
Original name
Taxonomic analysis and reclassification of Bifidobacterium adolescentis CCM 3761
Name in Czech
taxonomická analýza a reklasifikace Bifidobacterium adolescentis CCM 3761
Authors
ŠPANOVÁ, Alena (203 Czech Republic, guarantor), Jana KŘÍŽOVÁ (203 Czech Republic), Radka BURDYCHOVÁ (203 Czech Republic), Vladimír DRÁB (203 Czech Republic) and Bohuslav RITTICH (203 Czech Republic)
Edition
Praha, 10th International symposium on the genetics of industrial microorganisms, p. P046, 1 pp. 2006
Publisher
Mikrobiologický ústav AV ČR, Praha
Other information
Language
English
Type of outcome
Stať ve sborníku
Field of Study
Genetics and molecular biology
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/06:00031777
Organization unit
Faculty of Science
ISBN
80-239-7323-1
Keywords in English
Bifidobacterium; ARDRA; RAPD; rep-PCR; sequencing; species identification
Tags
International impact
Změněno: 2/1/2007 14:50, doc. Ing. Bohuslav Rittich, CSc.
V originále
DNA fingerprinting methods (ARDRA, RAPD, rep-PCR) have been applied for discrimination of Bifidobacterium species. Sixteen Bifidobacterium strains of eleven species which were used to evaluate the discrimination power of the above-mentioned methods were obtained from the Czech Collection of Microorganisms (CCM, Brno, Czech Republic), the American Type Culture Collection (ATCC, Manassas, USA), and the Deutsche Sammlung von Mikroorganismen und Zellkulturen (DSMZ, Braunschweig, Germany). In characterisation of the studied strains it was found that the results obtained for the strain B. adolescentis CCM 3761 did not correspond with its taxonomic position and the strain should be reclassified as B. pseudocatenulatum. The amplicons of the 16S rDNA region of B. adolescentis CCM 3761 and B. catenulatum CCM 7094, B. pseudocatenulatum DSM 16992T, and B. adolescentis CCM 4987T were used for sequencing of DNA and for confirmation of species identification.
In Czech
Typizační metody (ARDRA, RAPD, rep-PCR) byly použity pro rozlišení bifidobakterií. K ověření rozlišovací schopnosti výše uvedených metod byly použity sbírkové kmeny (CCM, ATCC, DSZM). Bylo zjištěno, že kmen Bifidobacterium adolescentis CCM 3761 neodpovídá jeho taxonomické pozici a kmen byl reklasifikován jako B. pseudocatenulatum. Amplikony z úseku 16S rDNA kmenů B. adolescentis CCM 3761,B. catenulatum CCM 7094, B. pseudocatenulatum DSM 16992T a B. adolescentis CCM 4987T byly použity k sekvencování DNA a potvrzení druhové identifikace.
Links
MSM0021622415, plan (intention) |
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QF3169, research and development project |
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