| MAZURA, Pavel, Radka FOHLEROVÁ, Břetislav BRZOBOHATÝ, Nagavalli Subbanna KIRAN and Lubomír JANDA. A new, sensitive method for enzyme kinetic studies of scarce glucosides. JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS. Elsevier, 2006, vol. 68, No 2006, p. 55-63. ISSN 0165-022X. |
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@article{707231,
author = {Mazura, Pavel and Fohlerová, Radka and Brzobohatý, Břetislav and Kiran, Nagavalli Subbanna and Janda, Lubomír},
article_number = {2006},
keywords = {Amplex UltraRed reagent; beta-glucosidase; glucose assay; glucose-oxidase / peroxidase coupled reaction; phytohormone conjugation; zeatin-O-glucoside},
language = {eng},
issn = {0165-022X},
journal = {JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS},
title = {A new, sensitive method for enzyme kinetic studies of scarce glucosides.},
volume = {68},
year = {2006}
}
TY - JOUR
ID - 707231
AU - Mazura, Pavel - Fohlerová, Radka - Brzobohatý, Břetislav - Kiran, Nagavalli Subbanna - Janda, Lubomír
PY - 2006
TI - A new, sensitive method for enzyme kinetic studies of scarce glucosides.
JF - JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
VL - 68
IS - 2006
SP - 55-63
EP - 55-63
PB - Elsevier
SN - 0165022X
KW - Amplex UltraRed reagent
KW - beta-glucosidase
KW - glucose assay
KW - glucose-oxidase / peroxidase coupled reaction
KW - phytohormone conjugation
KW - zeatin-O-glucoside
N2 - We have developed a rapid, accurate and sensitive assay for glucose that enabled us to measure the kinetic parameters for a cytokinin-O-glucoside-specific beta-glucosidase towards its natural substrate. Both the beta-glucosidase reaction and the detection reaction occur simultaneously in solution. Measurement is continuous and the reaction is measured nine times during three seconds.
ER -
MAZURA, Pavel, Radka FOHLEROVÁ, Břetislav BRZOBOHATÝ, Nagavalli Subbanna KIRAN and Lubomír JANDA. A new, sensitive method for enzyme kinetic studies of scarce glucosides. \textit{JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS}. Elsevier, 2006, vol.~68, No~2006, p.~55-63. ISSN~0165-022X.
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