MAZURA, Pavel, Radka FOHLEROVÁ, Břetislav BRZOBOHATÝ, Nagavalli Subbanna KIRAN and Lubomír JANDA. A new, sensitive method for enzyme kinetic studies of scarce glucosides. JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS. Elsevier, 2006, vol. 68, No 2006, p. 55-63. ISSN 0165-022X. |
Other formats:
BibTeX
LaTeX
RIS
@article{707231, author = {Mazura, Pavel and Fohlerová, Radka and Brzobohatý, Břetislav and Kiran, Nagavalli Subbanna and Janda, Lubomír}, article_number = {2006}, keywords = {Amplex UltraRed reagent; beta-glucosidase; glucose assay; glucose-oxidase / peroxidase coupled reaction; phytohormone conjugation; zeatin-O-glucoside}, language = {eng}, issn = {0165-022X}, journal = {JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS}, title = {A new, sensitive method for enzyme kinetic studies of scarce glucosides.}, volume = {68}, year = {2006} }
TY - JOUR ID - 707231 AU - Mazura, Pavel - Fohlerová, Radka - Brzobohatý, Břetislav - Kiran, Nagavalli Subbanna - Janda, Lubomír PY - 2006 TI - A new, sensitive method for enzyme kinetic studies of scarce glucosides. JF - JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS VL - 68 IS - 2006 SP - 55-63 EP - 55-63 PB - Elsevier SN - 0165022X KW - Amplex UltraRed reagent KW - beta-glucosidase KW - glucose assay KW - glucose-oxidase / peroxidase coupled reaction KW - phytohormone conjugation KW - zeatin-O-glucoside N2 - We have developed a rapid, accurate and sensitive assay for glucose that enabled us to measure the kinetic parameters for a cytokinin-O-glucoside-specific beta-glucosidase towards its natural substrate. Both the beta-glucosidase reaction and the detection reaction occur simultaneously in solution. Measurement is continuous and the reaction is measured nine times during three seconds. ER -
MAZURA, Pavel, Radka FOHLEROVÁ, Břetislav BRZOBOHATÝ, Nagavalli Subbanna KIRAN and Lubomír JANDA. A new, sensitive method for enzyme kinetic studies of scarce glucosides. \textit{JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS}. Elsevier, 2006, vol.~68, No~2006, p.~55-63. ISSN~0165-022X.
|