Detailed Information on Publication Record
2006
Identification of Lactobacillus spp. isolated from dental caries of children
ŠVEC, Pavel, Ivo SEDLÁČEK, Martina KUKLETOVÁ, Lenka ŽÁČKOVÁ, Jarmila KUKLOVÁ et. al.Basic information
Original name
Identification of Lactobacillus spp. isolated from dental caries of children
Name in Czech
Identifikace kmenů Lactobacillus spp. izolovaných ze zubního kazu dětí
Authors
ŠVEC, Pavel (203 Czech Republic, guarantor), Ivo SEDLÁČEK (203 Czech Republic), Martina KUKLETOVÁ (203 Czech Republic), Lenka ŽÁČKOVÁ (203 Czech Republic) and Jarmila KUKLOVÁ (203 Czech Republic)
Edition
2006
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10600 1.6 Biological sciences
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14110/06:00025581
Organization unit
Faculty of Medicine
Keywords (in Czech)
Lactobacillus; identifikace; taxonomie; dentalní plaque
Keywords in English
Lactobacillus; identification; taxonomy; dental plaque
Tags
Reviewed
Změněno: 31/3/2010 10:58, prof. RNDr. Ivo Sedláček, CSc.
V originále
Aim. To isolate and characterize Lactobacillus spp. inhabiting dental caries of children (up to age of 10). Methods. A total of 33 lactobacilli were isolated from 26 cases in period August to December 2005. Caries samples obtained from individual patients were cultivated in 5 % CO2 atmosphere on MRS and/or BHI agar supplemented by 10 % sheep blood. Biochemical and physiological properties of all strains were characterized by API 50CH kit and conventional tube tests. Rep-PCR fingerprinting with the (GTG)5 primer was used for genotype characterization of the isolates. Results. Evaluation of biochemical test results assigned 2 strains as L. acidophilus, 2 as L. casei, 2 as L. cellobiosus, 3 as L. fermentum, 1 as L. paracasei, 1 as L. plantarum, 5 as L. rhamnosus and 4 as L. salivarius; remaining strains (13) were not identified only to the species level. (GTG)5-PCR fingerprinting grouped the analysed strains into a few clusters in nearly full agreement with biotype results and clarified taxonomic position of 4 biochemically unidentified strains. Two unidentified groups of isolates were separated in the full correspondence by both biotyping as well as genotyping into two homogeneous clusters. These groups may represent novel taxa. Conclusion. A total of 8 Lactobacillus species were isolated from dental caries of children. Results obtained by both methods were comparable. However, (GTG)5-PCR fingerprinting has been shown as a quick, easy and less expensive identification tool than biotyping. Financial support from project No. 1M0021622409 is acknowledged.
In Czech
Práce charakterizuje druhy rodu Lactobacillus osídlující zubní kaz dětí.
Links
| MSM0021622416, plan (intention) |
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| 1M0528, research and development project |
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