Detailed Information on Publication Record
2006
ERIC-PCR fingerprinting of presumptive Aeromonas caviae strains isolated from surface waters
ŠVEC, Pavel, Dana NOVÁKOVÁ and Ivo SEDLÁČEKBasic information
Original name
ERIC-PCR fingerprinting of presumptive Aeromonas caviae strains isolated from surface waters
Name in Czech
ERIC-PCR typizace presupmtivních kmenů Aeromonas caviae izolovaných z povrchových vod
Authors
ŠVEC, Pavel (203 Czech Republic, guarantor), Dana NOVÁKOVÁ (203 Czech Republic) and Ivo SEDLÁČEK (203 Czech Republic)
Edition
2st FEMS Congress of European Microbiologists, 2006
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10600 1.6 Biological sciences
Country of publisher
Spain
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/06:00021835
Organization unit
Faculty of Science
Keywords in English
ERIC-PCR; Aeromonas caviae; identification; taxonomy; water
Tags
Změněno: 24/3/2010 13:38, doc. RNDr. Pavel Švec, Ph.D.
V originále
A group of 76 bacterial strains identified phenotypically as presumptive Aeromonas caviae was characterized by ERIC-PCR fingerprinting and compared to reference strains representing all described Aeromonas species. Biochemical traits revealed by environmental aeromonads were highly heterogeneous, however they matched the phenotypic criteria stated in the species description of A. caviae. All strains were typeable by ERIC primer and generated DNA fragments ranging from 200 to 3500 bp. Cluster analysis of ERIC-PCR fingerprints separated analysed strains into five clusters and grouped some of them with particular reference strains: A. caviae cluster (8 strains), A. hydrophila ssp. hydrophila / A. simiae cluster (11), A. media (13). Remaining two clusters (39 and 5 strains) were separated from all reference strains. All strains including selected representative strains grouped in individual clusters revealed typical visually very close basic fingerprint patterns implying that the biochemical identification of the particular strains as A. caviae is doubtful. Obtained results imply ERIC-PCR as a promising method applicable for fast, reliable and cheap screening of aeromonads isolated from the environment. Financial support from project MSM0021622416 is gratefully acknowledged.
In Czech
Práce popisuje využití metody ERIC-PCR pro identifikaci presumptivních kmenů Aeromonas cavie izolovaných z povrchových vod.
Links
| MSM0021622416, plan (intention) |
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