ŠVEC, Pavel, Dana NOVÁKOVÁ and Ivo SEDLÁČEK. ERIC-PCR fingerprinting of presumptive Aeromonas caviae strains isolated from surface waters. In 2st FEMS Congress of European Microbiologists. 2006.
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Basic information
Original name ERIC-PCR fingerprinting of presumptive Aeromonas caviae strains isolated from surface waters
Name in Czech ERIC-PCR typizace presupmtivních kmenů Aeromonas caviae izolovaných z povrchových vod
Authors ŠVEC, Pavel (203 Czech Republic, guarantor), Dana NOVÁKOVÁ (203 Czech Republic) and Ivo SEDLÁČEK (203 Czech Republic).
Edition 2st FEMS Congress of European Microbiologists, 2006.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 10600 1.6 Biological sciences
Country of publisher Spain
Confidentiality degree is not subject to a state or trade secret
RIV identification code RIV/00216224:14310/06:00021835
Organization unit Faculty of Science
Keywords in English ERIC-PCR; Aeromonas caviae; identification; taxonomy; water
Tags Aeromonas caviae, ERIC-PCR, identification, taxonomy, water
Changed by Changed by: doc. RNDr. Pavel Švec, Ph.D., učo 1098. Changed: 24/3/2010 13:38.
Abstract
A group of 76 bacterial strains identified phenotypically as presumptive Aeromonas caviae was characterized by ERIC-PCR fingerprinting and compared to reference strains representing all described Aeromonas species. Biochemical traits revealed by environmental aeromonads were highly heterogeneous, however they matched the phenotypic criteria stated in the species description of A. caviae. All strains were typeable by ERIC primer and generated DNA fragments ranging from 200 to 3500 bp. Cluster analysis of ERIC-PCR fingerprints separated analysed strains into five clusters and grouped some of them with particular reference strains: A. caviae cluster (8 strains), A. hydrophila ssp. hydrophila / A. simiae cluster (11), A. media (13). Remaining two clusters (39 and 5 strains) were separated from all reference strains. All strains including selected representative strains grouped in individual clusters revealed typical visually very close basic fingerprint patterns implying that the biochemical identification of the particular strains as A. caviae is doubtful. Obtained results imply ERIC-PCR as a promising method applicable for fast, reliable and cheap screening of aeromonads isolated from the environment. Financial support from project MSM0021622416 is gratefully acknowledged.
Abstract (in Czech)
Práce popisuje využití metody ERIC-PCR pro identifikaci presumptivních kmenů Aeromonas cavie izolovaných z povrchových vod.
Links
MSM0021622416, plan (intention)Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase
Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time
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